Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1994-10-06
1999-11-09
Hutzell, Paula K.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 71, 436518, 436811, G01N 3353
Patent
active
059812087
ABSTRACT:
A method useful in the diagnosis of Alzheimer's Disease in a patient in which an amyloid protein precursor (APP) substrate is combined with a sample of cerebrospinal fluid or blood obtained from the patient to be tested, and poteolytic cleavage of the APP substrate is detected. The absence of detectable proteolytic cleavage, or the detection of a substantially lesser degree of proteolytic cleavage, in the presence of the patient's sample compared to that detected when an APP substrate is combined with test samples from control individuals, indicates affliction with Alzheimer's Disease. Convenient test reagents and kits for aiding the diagnosis of Alzheimer's Disease are provided, such as comprising an APP substrate and immunoreagents for detecting a fragment formed by proteolytic cleavage as well as chromogenic APP substrates.
REFERENCES:
patent: 4874694 (1989-10-01), Gandy et al.
patent: 5039511 (1991-08-01), Quay et al.
patent: 5200324 (1993-04-01), Navaratnam et al.
patent: 5200339 (1993-04-01), Abraham
Kitaguchi et al Determination & Amyloid .beta. Protein Precensors Harboring, Active Form Proteinase Inhibitor Domains in Cerebrospinal Fluid of Alzheimers Disease Patents by Tryspin Antibody Sanduech Elisa vol. 166, No. 3 pp. 1453-1459 Feb. 14, 1990.
Schilling, James, "Recombinant Alzheimers Amyloid Protein" WO 90/14840 Published Dec. 13, 1990.
Morris et al The Consortuim to Establish a Registry for Alzheimer's Disease (CERAD) Sep. 1989 Neurology 39:1159-1165.
Matsubara et al Serum Concentration of .alpha., Antichymotrypsin in Patents With Senile Dementa of the Alzheimer's Type In: Iqbulk, et al eds. Alzheimers Disease and Related Disorders New York: Alan R. Liss 1989 :707-714.
Gollin et al., ".alpha.1 Antitrypsin and .alpha.1 Antichymotryspin are in the Lesins of Alzheimer's Disease" Neuro Report 3:201-203 Feb. 1992.
Abraham et al; Biochem Biophys Res Comm; 174 (2), 1991.
Bush et al; Jo Biol Chem, 265 (26) pp. 15977-15983, 1990.
Van Norstrand et al; Jo Biol. Chem, 265 (17) pp. 9591-9594, 1990.
Kang, The Precursor of Alzheimer's Disease Amyloid A4 Protein Resembles a Cell-Surface Receptor, Nature, vol.325:pp. 733-736, Feb. 19, 1987.
Wolozin et al., A Neuronal Antigen in the Brains of Alzheimer Patients, Science, vol.323:pp. 648-650 Feb. 18, 1986.
Bausch Kathryn M.
Dreyer Robert N.
Tamburini Paul P.
Bayer Corporation
Duffy Patricia A.
Hutzell Paula K.
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