Diagnostic and prognostic ELISA assays of serum .alpha.-N-acetyl

Details Diagnostic and prognostic ELISA assays of serum .alpha.-N-acetyl Diagnostic and prognostic ELISA assays of serum .alpha.-N-acetyl

1998-03-13

1999-11-16

Stucker, Jeffrey

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 71, 435 74, 435 79, 435 792, 435 793, 435 794, 435 795, 435974, 436518, 436531, C12Q 170

Patent

active

059855452

ABSTRACT:
HIV- virus-infected cells secrete .alpha.-N-acetylgalactosaminidase into the blood stream, resulting in the deglycosylation of the Gc protein. This inactivates the MAF precursor activity of the Gc protein, leading to immunosuppression. Thus, the .alpha.-N-acetylgalactosaminidase activity in a patient's bloodstream can serve as a diagnostic and prognostic index. Antibody-sandwich ELISA method and kits for HIV virus specific .alpha.-N-acetylgalactosaminidase as an antigen were developed to detect serum or plasma .alpha.-N-acetylgalactosaminidase activity in AIDS/HIV-infected patients and are used as a diagnostic/prognostic index.

REFERENCES:
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