Diagnostic and prognostic ELISA assays of serum .alpha.-N-acetyl

Details Diagnostic and prognostic ELISA assays of serum .alpha.-N-acetyl Diagnostic and prognostic ELISA assays of serum .alpha.-N-acetyl

1997-09-26

1998-07-07

Stucker, Jeffrey

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 79, 435 18, 435 34, 436531, C12Q 170

Patent

active

057766711

ABSTRACT:
Influenza virus-infected cells secrete .alpha.-N-acetylgalactosaminidase into the blood stream, resulting in the deglycosylation of the Gc protein. This inactivates the MAF precursor activity of the Gc protein, leading to immunosuppression. Thus, the .alpha.-N-acetylgalactosaminidase activity in a patient's bloodstream can serve as a diagnostic and prognostic index. Antibody-sandwich ELISA method and kits for influenza virus specific .alpha.-N-acetylgalactosaminidase as an antigen were developed to detect serum or plasma .alpha.-N-acetylgalactosaminidase activity in influenza infected patients and are used as a diagnostic/prognostic index.

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