Diagnostic and amplification methods using primers having thymin

Details Diagnostic and amplification methods using primers having thymin Diagnostic and amplification methods using primers having thymin

1989-09-12

1993-03-23

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 435805, 435948, 436501, 436811, 536 27, 935 6, 935 17, 935 19, 935 78, 935 88, C12Q 168

Patent

active

051963051

ABSTRACT:
Methods for amplifying and detecting a predetermined target nucleic acid in a biological specimen are accomplished even where there is a mismatch in a single position between a primer and the target nucleic acid. The mismatch is located at or near the 3' end of the primer. Such a mismatch is overcome using a primer having a nucleotide with a thymine base at the position of the mismatch. The use of such primers is most likely to prime the target and form primer extension products. This method is particularly useful for detection of a nucleic acid sequence which is not fully known, or where there is considerable heterogeneity in DNA target from patient samples.

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