Diagnostic agents

Details Diagnostic agents Diagnostic agents

1982-02-04

1983-11-08

Hruskoci, Peter A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

435 28, 435188, 435810, C12Q 160

Patent

active

044143265

ABSTRACT:
Diagnostic agents are described for determining high density lipoprotein cholesterol in body fuids such as serum or plasma. One is an enzymatic reagent for quantitative analysis for cholesterol composed of cholesterol oxidase, cholesterol esterase, peroxidase, 4-amino-antipyrine, a phenol or phenol derivative, a surfactant, such as sodium cholate and a polyglycol of molecular weight from about 190 to 1000, buffered to pH 5.5 to 7.8 in water. The other is a single, stable precipitating agent for lipoproteins in body fluids, comprising a bivalent metal salt of a monobasic acid, such as magnesium chloride and a water-soluble polyanion, such as sodium phosphotungstate in water at pH 5 to 8.

REFERENCES:
patent: 3062623 (1962-11-01), Schain
patent: 3907645 (1975-09-01), Richmond
patent: 3983005 (1976-09-01), Goodhue et al.
patent: 4089747 (1978-05-01), Bruschi
patent: 4161425 (1979-07-01), Perry
patent: 4164448 (1979-08-01), Roeschlau
patent: 4186251 (1980-01-01), Tarbutton
patent: 4226713 (1980-10-01), Goldberg
Allain et al., "Enzymatic Determination of Total Serum Cholesterol," Clinical Chemistry, vol. 20, 1974, pp. 470-475.

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