Diagnosis of Whipple's disease

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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C435S007320, C435S041000, C435S243000, C435S325000, C435S383000, C435S392000, C424S093100, C424S178100

Reexamination Certificate

active

07410787

ABSTRACT:
The invention relates to a method for in vitro seriological diagnosis of Whipple's disease, whereby the bacteria responsible for the disease are isolated and established in a culture and brought into contact with the serum or biological fluid of an infected patient. The invention also relates to useful oligonucelotides with a probe and a primer for amplifying, sequencing and detecting the gene rpoB of the bacteria,Tropheryma whippelii.

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Drancourt, “Tropheryma whippelii,pathogene emergent a culture intracellulaire responsible de la maladie de Whipple”, Presse Medicale, vol. 28, No. 8, Feb. 27, 1999, pp. 435-439.
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Zaaijer, H. L. et al., “De ziekte van Whipple,” Nederlands Tijdschrift Voor Geneeskunde, vol. 143, No. 8, pp. 388-392, 1999.
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Hinrikson, H. P., “Detection of three different types of ‘Tropheryma whippelii’ directly from clinical specimens by sequencing single-strand conformation polymorphism (SSCP) analysis and type specific PCR of their 16S-23S ribosomal intergenic spacer region,” International Journal of Systematic Bacteriology, vol. 49, pp. 1701-1706, 1999.

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