Diagnosis of Candida vaginitis by detecting D-arabinitol in vagi

Details Diagnosis of Candida vaginitis by detecting D-arabinitol in vagi Diagnosis of Candida vaginitis by detecting D-arabinitol in vagi

1993-11-10

1994-11-01

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 4, 435295, 435852, 435921, 436518, 436904, C12Q 132, C12N 100

Patent

active

053607180

ABSTRACT:
A method for the presumptive detection of Candida in vaginal fluid, thereby providing an aid to the diagnosis of Candida vaginosis. A sample of vaginal fluid is combined with a test composition comprising an indicator system that provides a chromogenic response in the presence of D-arabinitol; the detection of an elevated level of D-arabinitol indicating the presumptive presence of Candida. The test composition preferably comprises the enzyme D-arabinitol dehydrogenase, NAD, an electron transfer agent, and a chromogenic indicator.

REFERENCES:
Soyama et al, Clin. Chem. Acta, vol. 149, pp. 149-154, 1985.
Wong et al, J. Clin. Microbiol., vol. 26, No. 9, pp. 1670-1674, Sep. 1988.
Wong et al, J. Infectious Diseases, vol. 146, No. 3, pp. 353-359, Sep. 1982.

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