Diagnosing periodontal disease by measuring proteolytic activity

Details Diagnosing periodontal disease by measuring proteolytic activity Diagnosing periodontal disease by measuring proteolytic activity

1990-12-24

1992-05-26

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

424 2, 435 21, 435 23, 435 29, 4352524, C12Q 104, C12Q 102, G01N 3348, C12N 120

Patent

active

051167352

ABSTRACT:
A colorimetric assay for diagnosis of periodontal disease is carried out by using a chromogenic test substance which is hydrolyzed by trypsin-like enzymes produced by periodontopathogenic bacteria in a sample specimen of subgingival plaque to release a chromophore. The presence of periodontal disease is thus indicated by a color change. The results of this assay are reliable provided at least a minimum number of microorganisms, on the order of 1,000,000 to 10,000,000, are present in the specimen. This assay determines whether the specimen includes the requisite number of microorganisms so that false negative results are not occasioned as a result of inadequate specimen size. Enzymatic activity of microorganisms in the specimen, illustratively phosphatase or peptidase enzymes, indicates specimen size sufficiency by the release of a chromophore from a nonspecific chromogenic test substance.

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patent: 4568535 (1986-02-01), Loesche
patent: 4582795 (1986-04-01), Shibuya et al.
patent: 4591554 (1986-05-01), Koumura et al.
patent: 4603108 (1986-07-01), Bascomb
Ames Company, Chart of Instructions for Microstrix-3, Division of Miles Laboratories, Inc. 1976.

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