Diacylglycerol kinase isoforms epsilon and zeta and methods of u

Details Diacylglycerol kinase isoforms epsilon and zeta and methods of u Diacylglycerol kinase isoforms epsilon and zeta and methods of u

1997-04-22

1999-11-02

Jocobson, Dian C.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

4352523, 43525233, 435135, 4353201, 435194, 536 231, 536 232, 536 235, C12N 120, C07H 2104

Patent

active

059768754

ABSTRACT:
Two novel diacylglycerol kinase (DGK) isoforms are disclosed. The cDNA of one DGK isoform, designated DGK.epsilon., is about 2.6 kb in length. The DGK.epsilon. cDNA has an open reading frame encoding 567 amino acids and has a predicted molecular mass of 63 kDA. DAG kinase .epsilon. is highly selective for arachidonate-containing diacylglycerol (DAG) substrates. The cDNA of the second isoform, designated DGK .zeta., is 3.5 kb in length. The DGK.zeta. cDNA contains a single large open reading frame encoding a 928-amino acid protein with a predicted molecular mass of 103.9 kDa. An alternatively spliced muscle specific species of DGK .zeta., DGK.zeta.-2 is also disclosed and characterized. DGK.zeta. is localized to the nucleus. A lysine-rich region with homology to the MARCKS protein is shown to be necessary and sufficient to confer nuclear localization to DGK.zeta. via a protein kinase C phosphorylation.

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