Diacylglycerol acyltransferase genes, proteins, and uses...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

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Details

C435S069100, C435S419000, C435S468000, C435S007100, C536S023100

Reexamination Certificate

active

07429473

ABSTRACT:
The present invention relates to diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes and proteins that exhibit both long-chain acyltransferase and acetyltransferase activity. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type transferase. The present invention also relates to methods of using diacylglycerol acyltransferase genes and proteins, including in their expression in transgenic organisms and in the production of acetyl-glycerides in plant oils, and in particular seed oils.

REFERENCES:
patent: WO 00/36114 (2000-06-01), None
patent: WO 00/32756 (2000-08-01), None
Hobbs et al. (1999) FEBS Lett. 452: 145-149.
Bouvier-Nave et al. (2000) Eur. J. Biochem. 267: 85-96.
Routaboul et al. (1999) Plant Physiol. Biochem. 37: 831-840.
Zou et al. (1999) Plant J. 19: 645-653.
Jako et al. (2001), Plant Phys 126, 861-874.
Lin et al. (1999) J. Biol. Chem. 274:23276-23285.
Bus et al., Chemistry and Physics of Lipids, 16:123-132 (1976).
Fretzen, FETT—LIPID 100:161-166 (1998).

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