Diacylglycerol acyltransferase and its use to preferentially...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

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C435S254110, C435S255100, C435S252300, C435S254200, C435S006120, C435S254800, C435S069100, C435S320100, C536S023200

Reexamination Certificate

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10861616

ABSTRACT:
The gene for the diacylglycerol acyltransferase (DGAT) fromRicinus communisL., the castor plant, has been isolated, cloned, and used to transform other plants and micro-organisms. When the gene is expressed in a heterologous system, the corresponding DGAT protein is active and shows unusual and selective action for hydroxylated fatty acyl glycerides. DGAT carries out the final step in castor oil biosynthesis, and is believed to be largely responsible for many of the attributes of castor oil, making it an excellent candidate for industrial uses. This invention makes it possible to enhance the oil-producing capacity of other plants and micro-organisms.

REFERENCES:
patent: 6552250 (2003-04-01), Nykiforuk et al.
patent: 6559359 (2003-05-01), Laten
patent: 6583302 (2003-06-01), Erhan et al.
patent: 6620986 (2003-09-01), McKeon et al.
patent: 2003/0074695 (2003-04-01), Farese, Jr. et al.
He, X. et al., “Cloning and Characterization of a cDNA Encoding Diacylglycerol Acyltransferase from Castor Bean,” Lipids (2004) 39(2):1-8.

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