Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Reexamination Certificate
2008-07-28
2010-06-22
Kallis, Russell (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
C800S288000, C800S312000, C800S314000, C800S320100, C800S322000, C435S183000, C435S320100, C435S419000, C536S023100, C536S023200, C536S023700
Reexamination Certificate
active
07741532
ABSTRACT:
The invention provides diacylglycerol acyltransferase (DAGAT) proteins, wherein said proteins are active in the formation of triacylglycerol from fatty acyl and diacylglycerol substrates. In one aspect,Mortierella ramannianaDAGAT proteins have been isolated and have molecular weights of between approximately 36 and 37 kDa as measured by SDS-PAGE. The invention also provides novel DAGAT polynucleotide and polypeptide sequences and to methods of producing such polypeptides using recombinant techniques. In addition, methods are provided for using such sequences to alter triacylglycerol levels in plants and to treat diseases associated with altered DAGAT activity or expression.
REFERENCES:
patent: 5880334 (1999-03-01), Suzuki et al.
patent: 6051755 (2000-04-01), Zou et al.
patent: 6100077 (2000-08-01), Sturley et al.
patent: 6344548 (2002-02-01), Farese et al.
patent: 6444876 (2002-09-01), Lassner et al.
patent: 6822141 (2004-11-01), Lardizabal et al.
patent: 7041872 (2006-05-01), Lassner et al.
patent: 7355097 (2008-04-01), Zou et al.
patent: 7417176 (2008-08-01), Lardizabal et al.
patent: 2005/0193446 (2005-09-01), Zou et al.
patent: WO 98/55631 (1998-06-01), None
patent: WO 00/01713 (1999-06-01), None
Anderson et al., “Purification of diacylglycerol: acyltransferase from rat liver to near homegeneity”,Journal of Lipid Research, Abstract, 35:535-545, 1994.
Bell, et al., “Enzymes of glycerolipid synthesis in eukaryotes,”Annu. Rev. Biochem., 49:459-487, 1980.
Blum et al., “Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels,”Electrophoresis, 8:93-99, 1987.
Bouvier-Nave et al., “Expression in yeast and tobacco of plant cDNAs encoding acyl coA: diacylglycerol acyltransferase,”Eur. J. Biochem., 267:85-96, 2000.
Bradford, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding,”Anal. Biochem., 72:248, 1976.
Brindley, “Metabolism of triacylglycerols,” In:Biochemistry of Lipids, Lipoproteins and Membranes, Vance et al. (eds.), Elsevier, Amsterdam, pp. 171-203, 1991.
Broun et al., “Cataytic plasticity of fatty acid modification enzymes underlying chemical diversity of plant lipids,”Science, 283:1315-1317, 1998.
Cases et al., “Identification of a gene encoding an acyl CoA: diacylclycerol acyltransferase, a key enzyme in triacylglycerol synthesis”,Proc. Natl. Acad. Sci. USA, Abstract, 95(22):13018-13023, 1998.
Coleman et al., “Physiological and nutritional regulation of enzymes of triacylglycerol synthesis,”Annu. Rev. Nutr, 20:77-103, 2000.
Dahlqvist et al., Phospholipid: diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants,Proc. Natl. Acad. Sci. USA, 97:6487-6492, 2000.
Dahlqvist et al., “Selective channelling of unusual fatty acids into triacylglycerols,” In:Advances in Plant Lipid Research, Sanches, et al. (Eds.), Universidad de Sevilla, Seville, Spain, pp. 211-214, 1998.
Daum et al., “Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae,” Yeast, 14:1471-1510, 1998.
Delepelaire et al., “Lithium dodecyl sulfate/polyacrylamide gel electrophoresis of thylakoid membranes at 4° C.: characterizations of two additional chlorophyll a-protein complexes,”Proc. Natl. Acad. Sci. USA, 76:111-115, 1979.
EMBL Accession No. AA822348, Abstract, Feb. 1998.
EMBL Accession No. AA880703, Abstract, Mar. 1998.
EMBL Accession No. AA880955, Abstract, Mar. 1998.
EMBL Accession No. AT83514, Abstract, Aug. 1995.
EMBL Accession No. CEAF3384, Abstract, May 1997.
EMBL Accession No. HS84166, Abstract, Jul. 1995.
EMBL Accession No. SCYOR245c, Abstract, Jul. 1996.
Harwood, “Recent advances in the biosynthesis of plant fatty acids,”Biochim. Biophys Acta, 1301:7-56, 1996.
Hobbs et al., “Cloning of a cDNA encoding diacylglycerol acyltransferase fromArabidopsis thalianaand its functional expression,”FEBS Letters, 452:145-149, 1999.
Jako et al., “Seed-specific over-expression of anArabidopsiscDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight,”Plant Physiology, 126:861-874, 2001.
Jones et al., “Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases,”The Plant Cell, 7:359-371, 1995.
Kamisaka et al., “Activation of detergent-solubilized diacylglycerol acyltransferase by anionic phospholipids,”J. Biochem., 119:520-523, 1996.
Kamisaka et al., “Characterization of the diacylglycerol acyltransferase activity in the membrane fraction from a fungus,”Lipids, 28:583-587, 1993.
Kamisaka et al., “Purification and characterization of diacylglycerol acyltransferase from the lipid body fraction of an oleaginous fungus,”Journal of Biochemistry, Abstract, 6(121):1107-1114, 1907.
Kamisaka et al., “Purification and characterization of diacylglycerol acyltransferase from the lipid body fraction of an oleaginous fungus,”J. Biochem., 121:1107-1114, 1997.
Katavic et al., “Alteration of seed fatty acid composition by an ethyl methanesulfonate-induced mutation inArabidopsis thalianaaffecting diacylglycerol acyltranferase activity,”Plant Physiol, 108:399-409, 1995.
Knutzon et al., “Modification of brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene,”Proc. Natl. Acad. Sci. USA, 89:2624-2628, 1992.
Kwanyuen et al., “Isolation and purification of diacylglycerol acyltransferase from germinating soybean cotyledons,”BBA—Lipids and Lipid Metabolism, Elsevier Science, 877:238-245, 1986.
Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4,”Nature, 277:680-685, 1970.
Lardizabal et al., “Purification of a jojoba embryo wax synthase, cloning of its cDNA, and production of high levels of wax in seeds of transgenic arabidopsis,”Plant Physiol., 122:645-655, 2000.
Lewin et al., “Analysis of amino acid motifs diagnostic for the sn-glycerol-3-phosphate acyltransferase reaction,”Biochemistry, 38:5764-5771, 1999.
Little et al., “Solubilization and characterization of diacylglycerol acyltransferase from microspore-derived cultures of oilseed rape,”Biochemical Journal, 304:951-958, 1994.
Metz et al., “Purification of a jojoba embryo fatty acyl-coenzyme a reductase and expression of its cDNA in high erucic acid rapeseed,”Plant Physiol., 122:635-644, 2000.
Neuwald, “Barth syndrome may be due to an acyltransferase deficiency,”Current Biology, 7:465-466, 1997.
Nishizuka, “Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase C,”Science, 258:607-614, 1992.
Oelkers et al., “A lecithin cholesterol acyltransferase-like gene mediates diacylglycerol esterification in Yeast,”J. Biol. Chem., 275:15609-15612, 2000.
Ohlrogge et al., “Fatty acid syntesis: from Co2 to functional genomics,”Biochemical Society Transactions, 28(6):567-573, 2000.
Roesler et al., “Targeting of the arabidopsis homomeric acetyl-coenzyme a carboxylase to plastids of rapeseeds,”Plant Physiol., 113:75-81, 1997.
Routaboul et al., “The TAG1 locus of arabidopsis encodes for a diacylglycerol acyltransferase,”Plant Physiol. Biochem., 37:831-840, 1999.
Smith et al., “Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking Dgat,”Nature Genetics, 25:87-90, 2000.
Sorger et al., “Synthesis of triacylglycerols by the acyl-coenzyme A:diacyl-glycerol acyltransferase Dga1p in lipid particles of the yeastSaccharomyces cerevisiae,”J. of Bacteriology, 184(2):519-524, 2002.
Stobart et al., “Triacylglycerols are synthesized and utilized by transacylation reactions in microso
Hawkins Deborah
Lardizabal Kathryn Dennis
Thompson Gregory A.
Kallis Russell
Li Esq. Chunping
Monsanto Technology LLC
Sonnenschein Nath & Rosenthal LLP
LandOfFree
Diacylglycerol acyl transferase proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Diacylglycerol acyl transferase proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Diacylglycerol acyl transferase proteins will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4232312