Diabrotica toxins

Details Diabrotica toxins Diabrotica toxins

1994-08-26

1997-08-19

Chereskin, Che S.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

800250, 800DIG56, 514 12, 536 2371, 4351723, A01H 400, C12N 1532, C07K 14325

Patent

active

056591233

ABSTRACT:
New modified Cry proteins, particularly modified CryIIIA proteins, having significantly altered toxicity, and DNA sequences encoding these proteins, are designed. Analysis of solvent-accessible amino acid positions in domain II of the CryIIIA protein by alanine-scanning mutagenesis identified individual amino acids involved in corn rootworm toxicity. Random replacement of these amino acids identifies modified proteins with improved toxicity. A combination of all or most thus identified improved amino acids in a single protein yields modified CryIIIA proteins with significantly improved toxicity. Particularly regions protruding from the Cry molecule and located at the apex of the Cry proteins, were identified as involved in toxicity.

REFERENCES:
Perlak et al. (1993) Plant Molecular Biology vol. 22, pp. 313-321.
Adang et al (1993) Plant Molecular Biology, vol. 21, pp. 1131-1145.
Cunningham et al (1989) Science vol. 244, pp. 1081-1084.
Li et al. (1991) Nature vol. 353, pp. 815-821.
Widner et al (1990) Journal of Bacteriology, vol. 172, pp. 2826-2832.

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