Di(uridine 5′)-tetraphosphate and salts thereof

Details Di(uridine 5′)-tetraphosphate and salts thereof Di(uridine 5′)-tetraphosphate and salts thereof

2005-03-29

2005-03-29

Wilson, James O. (Department: 1623)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

C514S012200, C514S051000, C514S277000, C514S851000, C536S026100, C536S025600, C424S045000, C424S427000, C128S898000, C623S004100, C606S107000

Reexamination Certificate

active

06872710

ABSTRACT:
The present invention is directed to P1, P4-di(uridine 5′)-tetraphosphate, tetra-alkali metal salts such as tetrasodium, tetralithium, tetrapotassium, and mixed tetra-alkali metal cations thereof. The tetra alkali metal salts of P1, P4-di(uridine 5′)-tetraphosphate are water-soluble, nontoxic, and easy to handle during manufacture. These tetra-monovalent alkali metal salts are more resistant to hydrolysis than the mono-, di-, or tri-acid salts, therefore, they provide an improved stability and a longer shelf life for storage. The present invention also provides methods for the synthesis of P1, P4-di(uridine 5′)-tetraphosphate, and its pharmaceutically acceptably acceptable salts thereof, and demonstrates the applicability to the production of large quantities. The methods substantially reduce the time required to synthesize diuridine tetraphosphate, for example, to three days or less.

REFERENCES:
patent: 4136175 (1979-01-01), Rideout et al.
patent: 5032680 (1991-07-01), Kawai et al.
patent: 5292498 (1994-03-01), Boucher
patent: 5449664 (1995-09-01), Verheyden et al.
patent: 5596088 (1997-01-01), Boucher et al.
patent: 5607836 (1997-03-01), Boucher et al.
patent: 5628984 (1997-05-01), Boucher
patent: 5635160 (1997-06-01), Stutts, III et al.
patent: 5656256 (1997-08-01), Boucher et al.
patent: 5691156 (1997-11-01), Boucher et al.
patent: 5763447 (1998-06-01), Jacobus et al.
patent: 5789391 (1998-08-01), Jacobus et al.
patent: 5837861 (1998-11-01), Pendergast et al.
patent: 5900407 (1999-05-01), Yerxa et al.
patent: 5902567 (1999-05-01), Boucher
patent: 5935555 (1999-08-01), Stutts et al.
patent: 5958897 (1999-09-01), Jacobus et al.
patent: 5962432 (1999-10-01), LaCroix et al.
patent: 5968913 (1999-10-01), LaCroix et al.
patent: 5972904 (1999-10-01), Jacobus et al.
patent: 5981506 (1999-11-01), Jacobus et al.
patent: 6022527 (2000-02-01), Boucher et al.
patent: 6133247 (2000-10-01), Boucher et al.
patent: 6143279 (2000-11-01), Boucher et al.
patent: 6159952 (2000-12-01), Shaffer et al.
patent: 6258374 (2001-07-01), Friess et al.
patent: 6319908 (2001-11-01), Yerxa et al.
patent: 6548658 (2003-04-01), Yerxa
patent: 1043329 (2002-08-01), None
patent: 07-242685 (1995-09-01), None
patent: WO 9640059 (1996-12-01), None
patent: WO 9735591 (1997-02-01), None
patent: WO 9729756 (1997-08-01), None
patent: WO 9803177 (1998-01-01), None
patent: WO 9803182 (1998-01-01), None
patent: WO 9815835 (1998-04-01), None
patent: WO 9819685 (1998-05-01), None
patent: WO 9834593 (1998-08-01), None
patent: WO 9834942 (1998-08-01), None
patent: WO 9901138 (1999-01-01), None
patent: WO 9905155 (1999-02-01), None
patent: WO 9909998 (1999-03-01), None
patent: WO 9932085 (1999-07-01), None
patent: WO 9961012 (1999-12-01), None
patent: WO 0020430 (2000-04-01), None
patent: WO 0030629 (2000-06-01), None
patent: WO 0039145 (2000-07-01), None
patent: WO 0050024 (2000-08-01), None
patent: WO 0102416 (2001-01-01), None
Coste et al., “Non-adenylylated Bis(5′-nucleosidyl) Tetraphosphates Occur inSaccharomyces cerevisiaeand inEscherichia coliand Accumulate upon Temperature Shift or Exposure to Cadmium”,J. Biol. Chem.262(25):12096-12103 (1987).
Guranowski et al., “Synthesis of Diadenosine 5′,5′″-P1,P4-Tetraphosphate (AppppA) from Adenosine 5′-Phosphosulfate and Adenosine 5′-Triphosphate Catalyzed by Yeast AppppA Phosphorylase,”Biochemistry27:2959-2964 (1988).
Lobaton et al., “Diguansinetetraphosphatase from Rat Liver: Activity on Diadenosine Tetraphosphate and Inhibition by Adenosine Tetraphosphate,”Eur. J. Biochem. 50:495-501 (1975).
Ng, E. and Orgel, L., “The action of a water-soluable carbodiimide and adenosine-5′-polyphosphates,”Nucleic Acid Res. 15(8):3573-3580 (1987).
Olivier, K., et al., “Actue Safety and Effects on Mucociliary Clearance of Aerosolized Uridine 5′—Triphosphate + Amiloride in Normal Human Adults,”—Am. J. Respir. Crit. Care Med.154:217-223 (1996).
Rapaport et al., “HeLa cell DNA polymerase α is tightly associated with tryptophanyl-tRNA synthetase and diadenosine 5′,5′″-P1,P4-tetraphosphate binding activites,”Proc. Natl. Acad. Sci. 78(2):838-842 (1981).
Sillerno et al., “Dinucleasidetriphosphatase from Rat Liver,”Eur. J. Biochem. 76:331-337 (1977).
Vallejo et al., “Dinucleosidasetetraphosphatase in Rat Liver andArtemia Salina,”Biochim. Biophy. Acta438:304-309 (1976).

Affiliated with

Also associated with

Rating

Di(uridine 5′)-tetraphosphate and salts thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Di(uridine 5′)-tetraphosphate and salts thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Di(uridine 5′)-tetraphosphate and salts thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3387536