Devices for maintaining and growing human stem and/or hematopoie

Details Devices for maintaining and growing human stem and/or hematopoie Devices for maintaining and growing human stem and/or hematopoie

1995-06-07

1999-03-30

Beisner, William H.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

4352891, 4352972, 4353081, C12M 306

Patent

active

058888071

ABSTRACT:
Methods, compositions and devices are provided for the growth of human stem and/or hematopoietic cells in culture. Bioreactors are provided in which diverse cell types are simultaneously-cultured in the presence of appropriate levels of nutrients and growth factors substantially continuously maintained in the bioreactor while removing undesirable metabolic products. This simultaneous culture of multiple cell types successfully reconstructs hematopoietic tissue ex vivo. Optionally, at least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. The invention also allows for the separate maintenance of stromal and hematopoietic cells, and to allow for harvesting of both the adherent and non-adherent cells.

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