Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1995-05-26
1998-05-05
Stone, Jacqueline M.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
455 6, 455 691, 4551721, 4551723, 424520, 424425, 530350, 530397, 530303, 6048971, C12N 1517, C12N 500, C12N 2104, A61K 150
Patent
active
057473250
ABSTRACT:
The present disclosure relates to the application of genetic engineering to provide artificial .beta. cells, i.e. cells which can secrete insulin in response to glucose. This is achieved preferably through the introduction of one or more genes selected from the insulin gene, glucokinase gene, and glucose transporter gene, so as to provide an engineered cell having all three of these genes in a biologically functional and responsive configuration. Assays for detecting the presence of diabetes-associated antibodies in biological samples using these and other engineered cells expressing diabetes-associated epitopes are described. Also disclosed are methods for the large-scale production of insulin by perfusing artificial .beta. cells, grown in liquid culture, with glucose-containing buffers.
REFERENCES:
patent: 4195125 (1980-03-01), Wacker
patent: 4353888 (1982-10-01), Sefton
patent: 5002661 (1991-03-01), Chick et al.
patent: 5175085 (1992-12-01), Johnson et al.
patent: 5427940 (1995-06-01), Newgard
Harrison et al., "Insulin Action on Activity and Cell Surface Disposition of Human HepG2 Glucose Transporters Expressed in Chinese Hamster Ovary Cells," J. Biol. Chem., 265:5793-5801, 1990.
Iynedjian et al., "Molecular Cloning of Glucokinase cDNA," J. Biol. Chem., 252:6032-6038, 1987.
Magnuson and Shelton, "An Alternate Promoter in the Glucokinase Gene Is Active in the Pancreatic .beta. Cell," J. Biol. Chem., 264:15936-15942, 1989.
Shimizu et al., "Control of Glucose Metabolism in Pancreatic .beta.-Cells by Glucokinase, Hexokinase, and Phosphofructokinase," Diabetes, 37:1524-1530, 1988.
Cullen and Mains, "Posttranslational Processing of Transfected Mouse Pro-Adenocorticotropin/Endorphin in Rat Growth Hormone-Secreting Tumor Cells," Endocrinology, 125(4):1774-1782, 1989.
Santerre et al., "Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells," Proc. Natl. Acad. Sci. USA, 78(7):4339-4343, Jul., 1981.
Chen et al., "Regulation of .beta.-cell glucose transporter gene expression," Proc. Natl. Acad. Sci., 87:4088-4092, 1990.
Shibasaki et al., "Overexpression of glucose transporter modulates insulin biosynthesis in insulin producing cell line," FEBS Lett., 270(1,2):105-107, 1990.
Johnson et al., "Underexpression of .beta. Cell High Km Glucose Transporters in Noninsulin-Dependent Diabetes," Science, 250:546-549, 1990.
Orci et al., "Evidence that down-regulation of .beta.-cell glucose transporters in non-insulin-dependent diabetes may be the cause of diabetic hyperglycemia," Proc. Natl. Acad. Sci., 87:9953-9957, 1990.
Johnson et al., "The High Km Glucose Transporter of Islets of Langerhans Is Functionally Similar to the Low Affinity Transporter of Liver and Has an Identical Primary Sequence," J. Biol. Chem., 265(12):6548-6551, 1990.
Newgard et al., "Glucokinase and glucose transporter expression in liver and islets: implications for control of glucose homeostasis," Biochem. Soc. Trans., 18:851-853, 1990.
Newgard et al., "Analysis of glucokinase and glucose transporter gene products in islet, liver, and anterior pituitary cells and their role in glucose sensing," FASEB J. 4(7):A2008, Abstract #1827.
Matsutani et al., "Polymorphisms of GLUT2 and GLUT4 Genes," Diabetes, 39:1534-1542, 1990.
B.ae butted.kkeskov et al., "Antibodies to a 64,000 M.sub.r Human Islet Cell Antigen Precede the Clinical Onset of Insulin-dependent Diabetes," J. Clin. Invest., 79:926-934, 1987.
B.ae butted.kkeskov et al., "Identification of the 64K autoantigen in insulin-dependent diabetes as the GABA-synthesizing enzyme glutamic acid decarboxylase," Nature, 347:151-156, 1990.
Kaufman et al., "Brain Glutamate Decarboxylase Cloned in .lambda.gt-11: Fusion Protein Produces .gamma.-Aminobutyric Acid," Science, 232:1138-1140, 1986.
Johnson et al., "Inhibition of glucose transport into rat islet cells by immunoglobulins from patients with new-onset insulin-dependent diabetes mellitus," N. Engl. J. Med., 322(10):653-659.
Permutt et al., "Cloning and functional expression of a human pancreatic islet glucose-transporter cDNA," Proc. Natl. Acad. Sci., 86:8688-8692, 1989.
Iynedjian et al., "Differential expression and regulation of the glucokinase gene in liver and islets of Langerhans," Proc. Natl. Acad. Sci., 86:7838-7842, 1989.
Maclaren, N.K., "Perspectives in Diabetes: How, When, and Why to Predict IDDM," Diabetes, 37:1591-1593, 1988.
Thorens et al., "Cloning and Functional Expression in Bacteria of a Novel Glucose Transporter Present in Liver, Intestine, Kidney, and .beta.-Pancreatic Islet Cells," Cell, 55:281-290, 1988.
Selden et al., "Regulation of insulin-gene expression: Implications for gene therapy," N. Engl. J. Med., 317(17):1067-1076, 1987.
Kuwajima et al., "The glucose-phosphorylating capacity of liver as measured by three independent assays," J. Biol. Chem., 261(19):8849-8853, 1986.
Eisenbarth, G.S., "Type I Diabetes Mellitus: A Chronic Autoimmune Disease," N. Engl. J. Med., 314(21):1360-1368, 1986.
Mueckler et al., "Sequence and structure of a human glucose transporter," Science, 229:941-945, 1985.
Moore et al., "Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell. Intracellular storage, proteolytic processing, and secretion on stimulation," Cell, 35:5331-583, 1983.
Kanatsuna et al., "Immunoglobulin from insulin-dependent diabetic children inhibits glucose-induced insulin release," Diabetes, 32:520-523, 1983.
Whitesell et al., "Transport and Metabolism of Glucose in an Insulin-Secreting Cell Line, .beta.TC-1," Biochemistry (1991) 30(49):11560-11566. Published in USA.
Tal et al., "Glucose Transporter Isotypes Switch in T-Antigen-Transformed Pancreatic .beta. Cells Growing in Culture and in Mice," Mol. Cell. Biol. (Jan. 1992) 12(1):422-432. Published in USA.
Lacy et al., "Maintenance of Normoglycemia in Diabetic Mice by Subcutaneous Xenografts of Encapsulated Islets," Science (Dec. 20, 1991) 254:1782-1784. Published in USA.
Sullivan et al., "Biohybrid Artificial Pancreas: Long-Term Implantation Studies in Diabetic, Pancreatectomized Dogs," Science (May 3, 1991) 252:718-721. Published in USA.
Hughes et al., "Engineering of Glucose-Stimulated Insulin Secretion and Biosynthesis in Non-Islet Cells," Proc. Natl. Acad. Sci. USA (Jan. 1992) 89:688-692. Published in USA.
Hughes et al., "Expression of Normal and Novel Glucokinase mRNAs in Anterior Pituitary and Islet Cells," J. Biol. Chem. (Mar. 5, 1991) 266(7):4521-4530. Published in USA.
Berson et al., "Insulin-I.sup.131 Metabolism in Human Subjects: Demonstration of Insulin Binding Globulin in the Circulation of Insulin Treated Subjected," J. Clin. Invest., 35:170-190, 1956.
Bottazzo et al., "Islet-Cell Antibodies in Diabetes Mellitus with Autoimmune Polyendocrine Deficiencies," Lancet, 7884(II), 1279-1283, 1974.
Christie, Michael R. et al., "Cellular and Subcellular Localization of an M.sub.r 64,000 Protein Autoantigen in Insulin-Dependent Diabetes," J. Biol. Chem., 265(1):376-381, 1990.
Flier, Jeffrey S. et al., "Distribution of Glucose Transporter Messenger RNA Transcripts in Tissues of Rat and Man," J. Clin. Invest., 79:926-934, 1987.
Hellman et al., "Evidence for Mediated Transport of Glucose in Mammalian Pancreatic .beta.-Cells," Biochem. Biophys. Acta, 241:147-154, 1971.
Johnson, J. et al., "Inhibition of Rat Islet 3-0-Methyl-D-Glucose (30MG) Uptake by Serum of Rats and Humans with Autoimmune Diabetes," Annual Meeting Program Published in Diabetes, 1987.
Kanatsuna et al., "Block in Insulin Release from Column-Perifused Pancreatic .beta.-Cells Induced by Islet Cell Surface Antibodies and Complement," Diabetes 30(1):231-234, 1981.
Leary, "Researchers Closing In On a Large-Scale Test to Screen for Diabetes," New York Times, 19 Jun. 1990.
Lernmark, A., and S. Baekkeskov, "Islet Cell Antibodies-Theoretical and Practical Implications," Diabetologia, 21:431-435, 1981.
Maclaren, Noel Keith, and Shih-Wen Huang, "Antibody to Cultured Human Insulinoma Cells in Insulin-Dependent Diabetes," Lancet, 997-999, 1975.
Pozzilli, O. et al., "Monocional Antibodies Defined Abnormalities of T-Lymphocytes in Type I (Insulin-Dependent
Board of Regents , The University of Texas System
Stone Jacqueline M.
Twomey Patrick
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