Chemical apparatus and process disinfecting – deodorizing – preser – Control element responsive to a sensed operating condition
Patent
1995-05-26
1998-05-26
Pyon, Harold Y.
Chemical apparatus and process disinfecting, deodorizing, preser
Control element responsive to a sensed operating condition
422 67, 422104, 436174, 436179, 436180, 4352872, 4353091, G01N 102
Patent
active
057560501
DESCRIPTION:
BRIEF SUMMARY
This application is a 371 of PCT/RU94/00179, filed Aug. 5, 1994.
FIELD OF THE INVENTION
The present invention relates in general to biology and, more specifically, provides a method and a device for microdispensing of aqueous solutions of various substances to a carrier.
BACKGROUND OF THE INVENTION
One well-known method for microdispensing of aqueous solutions of substances onto a carrier uses rarefaction of the medium over the surface of the solution for filling a closed calibrated space, and excess pressure for ejecting said solution from the calibrated space back into solution or onto the surface of a carrier. A device for carrying said method into effect comprises a precision pair "cylinder-piston," wherein displacement of the piston with respect to the cylinder establishes rarefaction above the piston, thereby providing for filling the below-piston space with a microvolume of solution which is ejected from said space during the return piston stroke. An increased quantity of simultaneously prepared microvolumes is provided, using this method, by an increased number of parallel channels. The method under consideration and the device carrying it into effect are capable of microdispensing of the aqueous solution of substances in the range from tens of milliliters down to one microliter with an accuracy of 0.5% to 1% (cf. Cole-Parmer.RTM. micro and macropipettors; L-07839-51.div.L-07839-76). Multichannel devices (Chempette.RTM. multichannel pipettes; L-07915-60) provide for microdispensing of quantities from tens of milliliters down to 5 .mu.l, respectively, with an accuracy of 1.5 to 2%, the maximal number of parallel channels being 12.
The method discussed before and the device for its realization cannot be used for dispensing microvolumes lesser than one microliter. Moreover, said method and device have a rather low dispensing capacity (no more than 12 parallel channels).
Another method for microdispensing of aqueous solutions of substances onto a carrier is used in BIOMEK.RTM. 1000 Automated Laboratory Workstation (BECKMAN) and intended for high-quality transfer of clones, cell lines, bacteria, aqueous DNA solutions, etc. The method of microdispensing comprises use of a rodlike transferring element. The method consists in wetting the free end surface of the transferring element with an aqueous solution of substance to form a dose of said aqueous solution on said surface, followed by transferring said dose and bringing it in contact with the surface of the carrier. The method in question is based on the action of intermolecular interaction forces at the liquid-solid interface.
Also known is a device for carrying said method into effect.
The device comprises a plate-like base, one side of which carries 96 needle-like rods which are clamped thereto at one end, the other (butt) faces of said rods being coplanar (S91-8545-AP-20; 1991 Beckman Instrument Inc.; Bulletin No. 7883).
The layout of the rods on the plate corresponds exactly to the arrangement of the wells containing the solutions, on the tray. Two standard sizes of the rods are available, differing in the diameter of the end face (0.0015" tip pins P/N 372172; 0.060" tip pins - P/N 372173; S91-8545-AP-20; 1991 Beckman Instrument Inc., Bulletin No. 7883).
Microdoses of aqueous solutions of substances are transferred from the tray wells to the carrier as follows.
The plate carrying the rods are secured in a holder joined to the moving positioner head. By moving the head one positions the plate above the tray so that one rod is located over each well, whereupon the plate is moved down along the Z-axis. As a result, the rods dip into the tray wells to get wetted with the solutions contained therein. Thereupon the plate is moved up along the Z-axis, displaced spatially, and positioned above the carrier surface. Next the plate is moved down again along the Z-axis until the rod ends get in contact with the carrier surface, whereby the microdoses of the solutions of substances are transferred from the rod ends onto the carrier. Thereupon the rods are
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patent: 4635488 (1987-01-01), Kremer
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patent: 5223225 (1993-06-01), Gautsch
patent: 5436129 (1995-07-01), Stapleton
patent: 5443791 (1995-08-01), Cathcart et al.
Ershow Gennady Moiseevich
Kirillov Evgenii Vladislavovich
Mirzabekov Andrei Darievich
Pyon Harold Y.
University of Chicago
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