Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1979-05-21
1981-03-24
Warden, Robert J.
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
141 31, 422 61, 435 30, 435810, C12M 120
Patent
active
042581354
ABSTRACT:
An improved device for the study of biochemical or enzymatic reactions produced by living organisms is disclosed. The device comprises a sandwich of two plastic sheets sealed to each other, the said sandwich having a central open area with upstanding walls which define a central receptacle which is closed at its bottom by one of said plastic sheets. Internal of the sandwich are a plurality of radially-extending cavities providing individual reaction chambers, which are in communication with the central receptacle internal of the sandwich, as by means of capillaries of reduced cross-section with respect to the reaction chambers themselves. The reaction chambers have apertures therein, preferably at their radially-outwardly extending portions, to enable aerobic growth therein. The device also comprises a central cap which fits down snugly internal of the central receptacle and the walls of which correspond to the inside of the walls of the central receptacle and are preferably of a height which enables the cap to extend to the bottom thereof, thereby isolating whatever is in the reaction chambers from the central receptacle.
The central cap is structured and positioned, preferably within said central receptacle, so as to constitute a plunger or piston and create a plunger or piston effect upon being pushed downwardly within the corresponding "cylinder bore" of said central receptacle, thereby to distribute the sample into the individual reaction chambers.
REFERENCES:
patent: 3367841 (1968-02-01), Buissiere et al.
patent: 3690836 (1972-09-01), Buissiere et al.
patent: 3694320 (1972-09-01), Buissiere
patent: 3986534 (1976-10-01), Schmidt
patent: 4070248 (1978-01-01), Schmidt
Hueschen Gordon W.
Warden Robert J.
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