Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1978-10-30
1980-12-02
Warden, Robert J.
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
233 27, 356246, 422 61, 422 72, 422102, 435 30, 435 33, 435 34, 435292, 435293, 435299, 435300, C12M 120
Patent
active
042372348
ABSTRACT:
An improved device for the study of biochemical or enzymatic reactions produced by living organisms is disclosed. The device comprises a sandwich of two plastic sheets sealed to each other, the said sandwich having a central open area with upstanding walls which define a central receptacle which is closed at its bottom by one of said plastic sheets. Internal of the sandwich are a plurality of radially-extending cavities providing individual reaction chambers, which are in communication with the central receptacle internal of the sandwich, as by means of capillaries of reduced cross-section with respect to the reaction chambers themselves. The reaction chambers have apertures therein at their radially-outwardly extending portions to enable aerobic growth therein. The reaction chambers may be provided with sheets of absorbent material and/or color-producing reagents, which are preferably in said absorbent material. The device is also provided with means for positioning of the device on the axis of a horizontal centrifuge, so that samples may be placed therein through the central receptacle portion and readily distributed into the individual reaction chambers thereof by means of rotation on a standard centrifuge. The device also preferably comprises a central cap which fits down snugly internal of the central receptacle and the walls of which correspond to the inside of the walls of the central receptacle and are of a height which enables the cap to extend to the bottom thereof, thereby isolating whatever is in the reaction chambers from the central receptacle. The entire device, after centrifugation to distribute the sample into the individual reaction chambers, may have the central cap placed therein and in turn be placed into a Petri dish, preferably provided with a layer of absorbent material therein to provide necessary moisture, and incubated in usual manner to produce reactions and/or color reactions in the various reaction chambers for purposes of characterizing or identifying the specimen placed in the central receptacle for testing.
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Hueschen Gordon W.
Warden Robert J.
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