Device for use in the isolation of a biological material such as

Details Device for use in the isolation of a biological material such as Device for use in the isolation of a biological material such as

1997-05-27

2000-08-29

Warden, Jill

Chemical apparatus and process disinfecting, deodorizing, preser

Control element responsive to a sensed operating condition

422 58, 422100, 436180, B01D 2990, B01D 2900

Patent

active

061104280

DESCRIPTION:

BRIEF SUMMARY
BACKGROUND OF THE INVENTION

The present invention relates to the isolation of a biological material, for example nucleic acid, from a basic material containing said biological material.
An example of a method for the isolation of nucleic acid is known from U.S. Pat. No. 5,234,809. In the case of this method the basic material, a chaotropic fluid and silica particles are mixed, with the result that the nucleic acid is adsorbed on the silica particles. The silica particles are then separated from the fluid and treated with a buffered eluant, in which the nucleic acid is dissolved off the particles. With this method HIV tests, for example, can be prepared by isolating the nucleic acid from the basic material, which is blood.
The object of the present invention then is to provide a device which is suitable for carrying out the methods described and similar methods.


SUMMARY OF THE INVENTION

To this end, the device according to the invention is characterized by a container for holding a mixture of the basic material, a chaotropic fluid and a solid phase which binds the biological material; means for separating the solid phase with the biological material from the fluid bound thereto; and means for connecting the container to an inlet and outlet for washing fluid for washing the biological material bound to the solid phase, to an inlet for an eluant fluid, and to an eluate reservoir for collection of the eluant with the dissolved biological material.
If the solid phase which binds; the biological material consists of particle material, then it is advantageous if the means for separating the solid phase from the fluid are provided with a filter for allowing through the fluid and retaining the particle material.
The means for connecting the container to the inlet or outlet for eluant fluid and washing fluid and/or to the eluate reservoir are preferably provided with a shut-off element, which can be provided with, for example, a septum and an outlet channel for allowing through fluid from the separating means to the outlet, and also a hollow needle element connecting to the separating means, for piercing the septum in order to connect the separating means to the eluate reservoir.
Such a shut-off element is simple, user-friendly and reliable in operation.
In a preferred embodiment of the device according to the invention the container is provided with two sections lying one above the other and separated by a constriction, and is also provided with a supply element which can be connected to the inlet and is movable between a position above the constriction and a position connecting in a close fit to the constriction.
In this embodiment both compressed gas for discharging the sample fluid from the container and the washing fluid and the eluant fluid can be fed into the container by one and the same supply element. During the infeed of gas the supply element will be in the position above the constriction, following which the supply element is moved to the position connecting suitably to the constriction, so that only the bottom part of the container, containing the solid phase with the biological material bound thereto, need be flushed, with the result that less washing fluid is needed and there is less of a risk of contamination occurring.
In an alternative embodiment the shut-off element is a valve element designed with at least one non-return valve between inlet and container.
In a more advanced development thereof the valve element is provided with three non-return valves: the first-mentioned non-return valve in the connection to a compressed gas supply, a second non-return valve between the container and the separating means, and a third non-return valve in a connection between the separating means and the inlet for the washing fluid and the eluant.
Compared with the embodiment with one valve, this embodiment has the advantage that the washing fluid is introduced directly into the separating means, and the container therefore does not need to be washed, with the result that only a small amount of washing fluid

REFERENCES:
patent: 3647390 (1972-03-01), Kubodera et al.
patent: 4483920 (1984-11-01), Gillespie et al.
patent: 4746490 (1988-05-01), Saneii
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4956298 (1990-09-01), Diekmann
patent: 4995967 (1991-02-01), Van Driessche
patent: 5039488 (1991-08-01), Kohr
patent: 5053454 (1991-10-01), Judd
patent: 5073341 (1991-12-01), Hargreaves
patent: 5114858 (1992-05-01), Williams et al.
patent: 5139637 (1992-08-01), MacConnell
patent: 5208160 (1993-05-01), Kikyotani et al.
patent: 5234809 (1993-08-01), Boom et al.
patent: 5284940 (1994-02-01), Lin et al.
patent: 5316731 (1994-05-01), Schrenk et al.
patent: 5330914 (1994-07-01), Uhlen et al.
patent: 5330916 (1994-07-01), Williams et al.
patent: 5346999 (1994-09-01), Cathcart et al.
patent: 5352609 (1994-10-01), Boquet
patent: 5358691 (1994-10-01), Clark et al.
patent: 5417924 (1995-05-01), Di-Martino et al.
patent: 5443743 (1995-08-01), Fetner et al.
patent: 5451528 (1995-09-01), Raymoure et al.
patent: 5552325 (1996-09-01), Nochumson et al.
patent: 5578459 (1996-11-01), Gordon et al.
patent: 5578495 (1996-11-01), Wilks
patent: 5593290 (1997-01-01), Greisch et al.
patent: 5599504 (1997-02-01), Hosoi et al.
patent: 5603845 (1997-02-01), Holm
patent: 5645723 (1997-07-01), Fujishiro et al.
patent: 5693785 (1997-12-01), Woodard et al.
patent: 5746978 (1998-05-01), Bienhaus et al.
Belgrader et al. Biotechniques 19 (1995) No. 3, 426-428.
Peyman et al. BioTechniques 16 (1994) No. 2, 210.
Lear et al. Am Biotechnol. Lab. 13 (1995) No. 3, 100.
Derwent Abstract No. 95-382762 (1994).
Derwent Abstract No. 94-293557 (1985).
Derwent Abstract No. 95-044223 (1993).
Derwent Abstract No. 95-090549 (1993).
Derwent Abstract No. 95-200378 (1993).
Derwent Abstract No. 95-206804 (1993).
Derwent Abstract No. 95-240775 (1993).
Derwent Abstract No. 95-241774 (1993).
Derwent Abstract No. 95-274924 (1992).
Derwent Abstract No. 95-370471 (1994).
Derwent Abstract No. 96-133418 (1994).
Derwent Abstract No. 96-160299 (1994).
Derwent Abstract No. 96-179893 (1994).
Derwent Abstract No. 95-190163 (1993).
Derwent Abstract No. 96-031612 (1994).
Derwent Abstract No. 95-255056 (1994).
Derwent Abstract No. 95-366254 (1994).
Derwent Abstract No. 95-366265 (1994).
Derwent Abstract No. 94-294348 (1993).
Derwent Abstract No. 95-051801 (1993).
Derwent Abstract No. 95-283025 (1993).
Derwent Abstract No. 96-031615 (1994).
Derwent Abstract No. 95-016567 (1993).
Derwent Abstract No. 94-237590 (1992).
Derwent Abstract No. 95-058795 (1991).
Derwent Abstract No. 95-188000 (1993).
Derwent Abstract No. 95-234336 (1993).
Derwent Abstract No. 96-106044 (1994).
Derwent Abstract No. 96-188389 (1994).
Derwent Abstract No. 95-148670 (1993).
Derwent Abstract No. 95-148671 (1993).
Derwent Abstract No. 95-329874 (1994).
Derwent Abstract No. 96-078605 (1994).
Derwent Abstract No. 96-187701 (1989).

Affiliated with

Also associated with

Rating

Device for use in the isolation of a biological material such as does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Device for use in the isolation of a biological material such as, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Device for use in the isolation of a biological material such as will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1246481