Fluid handling – With indicator – register – recorder – alarm or inspection means – Fluid pressure responsive indicator – recorder or alarm
Patent
1985-06-07
1989-06-06
Hepperle, Stephen
Fluid handling
With indicator, register, recorder, alarm or inspection means
Fluid pressure responsive indicator, recorder or alarm
137597, 137599, 251295, 604118, F17D 305
Patent
active
048362441
ABSTRACT:
A method and a device are proposed for the injection of very small amounts of samples into cells (74) with the aid of a microcapillary (10). During the injection, a constant injection pressure of about 0.12 bar, provided by a pressurized cylinder (46) is applied ot the microcapillary (10), which is reduced after the completion of the injection to a holding pressure of 0.02 bar until the next cell (64) is to be injected. A high-pressure (p.sub.1) supplied from another pressurized cylinder (44) serves for blowing out the microcapillary tip (12). A three-way valve (42) permits simple and rapid switching between the various pressure (p.sub.1, p.sub.2 and p.sub.3). Reproducible amounts of sample in the range from 10.sup.-11 ml to 10.sup.-13 ml can be injected reprodicibly, simply and rapidly.
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patent: 3477467 (1969-11-01), Sewell et al.
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Graessmann et al, Methods in Enzymology, vol. 65, (1980), pp. 816-825.
Stacey, "Microinjection of mRNA and other Macromolecules into Living Cells" in Buch, Methods in Enzymology, vol. 79B, pp. 76-88, (1981).
Chem. Abstracts 92:193643t, (1980).
Chem. Abstracts 94:1675b, (1981).
Mueller et al-Trends Biochem. Sci. (Pers. Ed.), (1980, vol. 5, No. 3, pp. 60-62.
Europaisches Laboratorium fur Molekularbiologie
Hepperle Stephen
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