Device for the elution of electrically-charged macromolecules

Details Device for the elution of electrically-charged macromolecules Device for the elution of electrically-charged macromolecules

1985-05-03

1988-02-16

Tung, T.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041828, G01N 2728, G01N 3368

Patent

active

047253487

ABSTRACT:
The present invention provides a device for the elution of electrically-charged macromolecules, especially biopolymers, from a gel by electrophoretic transfer of the macromolecules from a gel placed under a buffer into a salt solution, said device having a container (2) for the reception of an electrolyte, which chamber is divided up by at least one reception chamber for a piece of gel, which reception chamber is permeable to the electrolyte, into two electrode chambers (12, 14), each of which contains at least one electrode (8, 10), wherein the electrode chambers (12, 14) are separated from one another by a block (4) provided with a closable electrolyte passage (6) and the at least one reception chamber for a piece of gel is formed by a trough (16) let into the surface of the block (4), which trough is connected via at least one first opening (20), present in or next to the bottom (18) thereof, with one electrode chamber (14) and via a second opening (22), present in or next to the bottom thereof with a canal (24), which first descends and then rises, opening into the other electrode chamber (12) for the reception of the salt solution.

REFERENCES:
patent: 3989612 (1976-11-01), Kragt et al.
patent: 4049534 (1977-09-01), Posner
patent: 4576702 (1986-03-01), Peck et al.
Diekmann, S., "A Device to Elute Biomolecules Out of Gels", Electrophoresis 84, Editor: Volker Neuhoff, pp. 154-155, Jul. 1984.

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