Device for the determination of incisional wound healing ability

Surgery – Truss – Pad

Patent

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A61B 500

Patent

active

051138719

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BRIEF SUMMARY
The present invention relates to a device and a method for the determination of the healing ability of an incisional wound or a connective tissue in man.
The tissue healing process begins within a matter of seconds from receiving a lesion or starting an operation and continues via blood coagulation and a highly diversified biological reaction chain towards connective tissue cicatrization. What in the beginning is a cell-abundant, slack and mechanically unstable tissue turns into a firmer and firmer tissue as days and weeks pass. Little by little the metabolism of this granulation tissue becomes slower. The shape, final size and microscopic texture of a cicatrix tissue are determined according to the patient's age, sex, general metabolism and local tissue strength requirement.
With animal experiments it has been possible to indicate that the amount of cells appearing in the wound area during the first days and the relative quantitative proportions thereof determine the course of healing for weeks onwards. Although it is possible with test animals to study the local healing rate of tissues in many different ways from the wound itself, this has not been possible with human beings. Clinically estimated, the healing of tissues is either a success or a failure. No information has been obtainable from a closed incisional wound about the deceleration of healing and the reasons possibly contributing to this.
There is a prior known device and method for collecting wound cells from an incisional wound (Viljanto, J., J. Surg. Res. 20 (1979) p. 115-119). In this prior
known method a thin silicone rubber tube with a cellulose sponge at one end is placed in the wound for picking up a sample of wound cells for analysis. An object of the present invention is to improve this device and method for providing a reliably operating, tissue-healing testing device as well as a method which is reliably reproducible and whereby the accuracy of analysis results is improved and their utilization is facilitated.
A device of the present invention for detecting wound healing ability comprises a flexible capillary tube inserted in a conventional manner into a wound, the tip of this tube left in the wound being fitted with a sponge for the attachment and growth of cells. A characterizing feature of this device of the invention is that at least that end of a capillary tube left inside the wound is provided with at least one inner groove and that the sponge is a wet-expanding viscose cellulose sponge, containing macro- and micropores in communication with each other. The inner design of a capillary tube according to the invention provides a firm attachment of the sponge to the end of a tube and at the same time there is secured the free entrance and movement for cell-containing wound exudate in the capillary tube and in the sponge. In a preferred embodiment, the interior of a capillary tube is divided into four substantially equal-sized grooves. This produces two opposite pairs of grooves having therebetween ridges retaining the piece of sponge.
The viscose cellulose sponge is preferably rectangular in cross-section and so dimensioned that, when dry, it extends from one groove of a capillary tube to the opposite groove, the side grooves remaining free even in the expanded condition of a viscose sponge, whereby the fluid is allowed a free flow in these free side grooves. The capillary tube is preferably made of silicone rubber.
The invention relates also to a method for the determination of incisional wound healing ability, wherein a sponge mounted on a capillary tube and intended for the attachment and growth of wound cells is inserted in a wound for sampling. This invention is characterized in that, after the sampling, the sponge is rinsed with a certain rinsing liquid, at a certain rinsing speed and with a certain amount of rinsing liquid, followed by treating the cell suspension in a per se known manner for differential counting of the cells and and comparing the obtained results with reference values.
The comparison is most preferably ef

REFERENCES:
patent: 1596754 (1926-08-01), Moschelle
patent: 3640268 (1972-02-01), Davis
patent: 3688763 (1972-09-01), Cromarty
patent: 3957054 (1976-05-01), McFarlane
Viljanto, A., Cellstu: A Device for Wound Healing Studies in Man, Journal of Surgical Research, 20:115-19 (1976), on or before Dec. 1976.

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