Device for the detection of selected motile organisms

Details Device for the detection of selected motile organisms Device for the detection of selected motile organisms

1990-04-23

1992-07-21

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

435287, 435284, 435291, 435299, 435300, C12M 140, C12M 116, C12M 118

Patent

active

051322293

ABSTRACT:
A device for detecting the presence of a particular motile organism within a sample are disclosed. The sample may be derived from dry milk, raw meat, poultry or a clinical specimen. A preferred method of using the device includes inoculating a selective enrichment medium containing a chemotactic attractant with the sample and contacting the selective enrichment medium with a nonselective motility medium containing a chemotactic attractant in a concentration less than the attractant concentration in the selective enrichment medium. Upon incubation, the motile organism metabolizes the chemotactic attractant, allowing the organism to move into the motility medium where it interacts with antibodies specific for the organism, thereby causing the formation of a persistent immobilization band. The device is particularly useful in detecting Salmonella.

REFERENCES:
patent: 4563418 (1986-01-01), Ward, Jr.

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