Device for sequential protein transfer from a gel

Details Device for sequential protein transfer from a gel Device for sequential protein transfer from a gel

2007-07-31

2007-07-31

Noguerola, Alex (Department: 1753)

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

C204S464000, C204S613000, C204S614000

Reexamination Certificate

active

10888075

ABSTRACT:
An apparatus and method for the sequential electroelution of biomolecules is described, the apparatus comprising a separation medium having an outlet, and a collector having at least a first receptacle and a second receptacle that can be sequentially brought into contact with the outlet of the separation medium by translating the first receptacle and the second receptacle in relation to the outlet of the separation medium, and the method comprising the steps of receiving a first substantially separated molecule in the first receptacle and translating the first receptacle and the second receptacle such that the second receptacle is brought into in contact with the outlet of the separation medium, receiving a second substantially separated molecule in the second receptacle, and repeating said steps to sequentially receive a desired number of substantially separated molecules.

REFERENCES:
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patent: 6001233 (1999-12-01), Levy
patent: 2002/0146839 (2002-10-01), Guttman et al.
Buzás et al.,An electroelution apparatus for sequential transfer of sodium dodecyl sulfate-proteins into agarose and mass spectrometric identification of Li-Na-dodectl sulfate-proteins from solubilized agarose, 25 Electrophoresis 966 (2004).
Buzás et al.,Direct vertical electroelution of protein from a PhastSystem band for mass spectrometric identification at the level of a few picomoles, Proteomics 2001, 1, 691-698.
Invitrogen Life Technologies, Instruction Manual, “XCellSureLock™ Mini-Cell, For leak-free electrophoresis of mini-gels,” Catalog Nos. E10001, E10020, E10002, Version H, Aug. 20, 2002,IM-9003, pp. i-22.
Radko et al.,Electroelution without gel sectioning of proteins from sodium dodecyl sulfate-polyacrylamide gel electrophoresis: Fluorescent detection, recovery isoelectric focusing and matrix assisted laser desorption/ionization-time of flight of the electroeluate, 23 Electrophoresis 985 (2002).
Yefimov et al.,Transfer of SDS proteins from gel electrophoretic zones into mass spectrometry, using electroelution of the band into buffer without sectioning of the gel, J. Biochem. Biophys. Methods 42 (2000) 65-78.

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