Device for separating magnetically labelled cells

Chemical apparatus and process disinfecting – deodorizing – preser – Control element responsive to a sensed operating condition

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210222, 210695, 2092231, 2092232, 209636, 422 99, 435 2, 435 723, 436526, B07C 5344

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active

055143407

ABSTRACT:
A device for separating magnetically labelled cells in a sample using an applied magnetic field having a housing; an inlet element at the top portion of the housing having an input end and an output end; a filter chamber adjacent to the output end of the inlet element for filtering the magnetically labelled cells from the fluid while allowing unlabelled cells to pass through when a magnetic field is applied thereto, and containing a multiplicity of magnetic matrix elements extending transversely across the filter chamber; and an outlet element for collecting the fluid which passes through the filter chamber having an input end coupled to the filter chamber and an output end. The device has one or more of the following features: (i) the inlet and/or outlet element having flow distribution means for distributing the flow from the inlet element generally uniformly across the filter chamber; (ii) the peripheries of the magnetic matrix elements are connected to the housing by a junction which is substantially impenetrable to the fluid; and (iii) the magnetic matrix elements are ordered and spaced apart so as to maximize the magnetic capture of magnetically labelled cells onto the magnetic matrix element when a magnetic field is applied. Methods of using the device and a hematopoietic cell preparation obtained using the device of the invention are also described.

REFERENCES:
patent: 2959287 (1960-11-01), Davis et al.
patent: 3136720 (1964-05-01), Baermann
patent: 3869390 (1975-03-01), Heitmann et al.
patent: 4025432 (1977-05-01), Nolan et al.
patent: 4190524 (1980-02-01), Watson
patent: 4209394 (1980-06-01), Kelland
patent: 4211658 (1980-07-01), McDonald et al.
patent: 4452773 (1984-06-01), Molday
patent: 4460463 (1984-07-01), Yoshida
patent: 4505824 (1985-03-01), Akamine et al.
patent: 4544482 (1985-10-01), Rupp
patent: 4737294 (1988-04-01), Kukuck
patent: 4868109 (1989-09-01), Lansdorp
patent: 5092987 (1992-03-01), Schickel
Perry et al. Chemical Engineer's Handbook McGraw-Hill, N.Y. (1973) pp. 5-47 to 5-49.
Bieva et al., Exp. Hematol., 17:914-920, 1989.
Miltenyi et al., Cytometry, 11:231-238, 1990.
Kogler et al., Bone Marrow Transplant., 6:163-168, 1990.
Thomas et al., J. Hematother., 2:297-303, 1993.
Yau et al., Exp. Hematol., 18:219-222, 1990.
Poynton et al., The Lancet, Mar., p. 524, 1983.
Reading et al., Leukemia Res., 11(12):1067-1077, 1987.
Kato, K., and Radbruch, A., Cytometry, 14:384-392, 1993.
Molday, R. S. and Molday, L., FEBS. Lett. 170(2):232-238, 1984.
Kemshead, J. T., J. of Hematotherapy, 1:35-44, 1992 at pp. 36 to 39.
Kemshead, J. T. The Immunomagnetic Manipulation of Bone Marrow in Bone Marrow Processing and Purging, 293, Gee, A. P. Ed., C.R.C. Press, Inc., Boca Raton, Florida, 1991.
Lansdorp et al., J. Exp. Med., 178:787-791, 1993.
Rebel et al., Blood, 83(1):128-136, 1994.
Ziolo et al., Science 257:219-223, 1992.
Molday, R. S. and MacKenzie, D., J. Immunol. Methods 52:353-367, 1982.
Staerz & Bevan, PNAS (USA) 83:1453-1457, 1986.
Staerz & Bevan, Immunology Today, 7(7&8):241-245, 1986.
Staerz et al., Nature, 314:628-631, 1985.
Perez et al., Nature 316:354-356, 1985.
Hoffmann, R. A. et al., Proc. Natl. Acad. Sci. U.S.A. 77(8):4914-4917, 1980.
Caldwell, C. W. and Taylor, H. M. Am. J. Clin. Path. 86:600, 1986.
Wognum et al., Cytometry 8:366-371, 1987.
Spangrude and Scollay, Exp. Hematol. 18:920-926, 1990.
Patent Abstracts of Japan, 23 Feb. 1988, vol. 12, No. 59(C-478) 2906! Diado Steel Co. Ltd, Multi-Flow Velocity Magnetic Filter, JP-A-62 204819, Sep. 1987.

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