Chemistry: electrical and wave energy – Apparatus – Electrophoretic or electro-osmotic apparatus
Patent
1998-06-09
2000-09-05
Warden, Sr., Robert J.
Chemistry: electrical and wave energy
Apparatus
Electrophoretic or electro-osmotic apparatus
204615, G01N 2726
Patent
active
061137669
ABSTRACT:
The present invention involves a gel strip carrier module for a gel strip that reduces the handling of the gel strip and the hands-on-time during preparation of the gel strip for isoelectric focusing. The gel carrier module includes a gel strip chamber that serves as both a rehydration and focusing chamber, and allows the sample to be applied either throughout the entire gel or in a defined zone. The gel carrier module includes a pair of electrodes near opposite ends of the chamber that the gel strip rests on, gel side facing down. The gel carrier module includes a cover with hold-down blocks or pressure blocks to assure reliable, light contact between the gel and the electrodes during focusing. A rehydration buffer is added into the chamber, and the gel strip is gently placed in the chamber, gel side down, for rehydration. The rehydration buffer may include the sample, or, in the event that the sample needs to be applied after rehydration, the gel carrier module includes sample application wells between the electrodes that the sample can be added to after rehydration of the gel strip.
REFERENCES:
patent: 4999340 (1991-03-01), Hoffman et al.
patent: 5209831 (1993-05-01), MacConnell
patent: 5399255 (1995-03-01), Sarrine
Immobiline.RTM. DryStrip Reswelling Tray: User Maual Amersham Pharmacia Biotech (1998).
"Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH gradients in the first dimension," Electrophoresis 15:1552-1558 (1994).
"Improved and simplified in-gel sample application using reswelling of dry immobilized pH gradients," Electrophoresis 18:324-327 (1997).
Hungerman Eric R.
Islam Mohammed Rezaul
Steiner Urs
Hoefer Pharmacia Biotech, Inc.
Malia Victoria M.
Noguerola Abe
Ronning, Jr. Royal N.
Warden, Sr. Robert J.
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