Device for preparing specimens for a cryo-electron microscope

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing a carotene nucleus

Reexamination Certificate

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C435S287100, C435S040500, C422S091000, C422S105000

Reexamination Certificate

active

07413872

ABSTRACT:
The invention relates to a device for preparing specimens for a cryo-electron microscope, comprising an environmental chamber, a holder for a sample or a carrier, and at least one blotting element to which a medium for absorbing liquid is or can be attached, both disposed in the environmental chamber, and a cooling medium for cooling down said sample. The said blotting element can be moved towards the sample or carrier in a controlled manner.

REFERENCES:
patent: 2680371 (1954-06-01), Donath
patent: 4753887 (1988-06-01), Bellare et al.
Trachtenberg, S. “A Fast-Freezing Device with a Retractable Environmental Chamber, Suitable for Kinetic Cryo-Electron Microscopy Studies”, Journal of Structural Biology, 1998, Academic Press, 123, pp. 45-55.
“A Fast-Freezing Device with a Retractable Environmental Chamber, Suitable for Kinetic Cryo-Electron Microscopy Studies”, by S. Trachtenberg, Journal of Structural Biology, vol. 123, 1998, pp. 45-55.
“Particle-surface interaction in thin vitrified films for cry-electron microscopy”, by M. Cyrklaff et al., Journal of Microscopy, vol. 175, No. 2, Aug. 1994 pp. 135-142.
“Cryoplunge”, retrieved from the Internet on Jul. 2002, http://www.gatan.com/pdf/Cryoplunge.pdf.

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