Device for performing qualitative enzyme immunoassays

Chemistry: molecular biology and microbiology – Apparatus – Bioreactor

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Details

435287, 435810, 426535, 426808, 426818, 422 57, 422 58, 422 61, 422 68, 422100, C12M 100, C12M 124, G01N 148, G01N 33544

Patent

active

047910607

ABSTRACT:
The invention relates to a device and method for performing qualitative enzyme immunoassays. The device comprises at least one tube having antibody, antigen or hapten attached to an internal surface thereof. A first syringe is connectable to a first end of the tube or tubes and supplies a flow of test liquid therethrough. A second syringe having a two piston arrangement is connected to the tube so as to allow a first wash solution followed by an enzyme conjugate solution to be flowed therethrough. Finally a third syringe, also having a two piston configuration, is connected to the tube so as to allow a second wash solution followed by an enzyme substrate and metabolite indicator solution to be flowed therethrough.

REFERENCES:
patent: Re31006 (1982-08-01), Schuurs et al.
patent: 4276048 (1981-06-01), Leaback
patent: 4585623 (1986-04-01), Chandler
patent: 4665034 (1987-05-01), Chandler

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