Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Patent
1997-08-01
1999-06-29
Beisner, William H.
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
4352879, 4352881, 4353061, 4353091, C12M 140
Patent
active
059168021
ABSTRACT:
A method of taking a sample of an inoculated culture medium and measuring the amount of ATP using a luciferase/luciferin reaction. Additional samples are taken at later time intervals to determine whether anything is growing in the medium. Luciferase/luciferin reagent is immobilized on an absorbent tip of a sampling device in order to stabilize the luciferase/luciferin reagent and to provide a convenient means to combine the luciferase/luciferin reagent with the sample. A counting tube containing an extraction reagent is employed to extract intracellular ATP from the sample. Chlorhexadine diacetate (CDA) is the preferred extraction reagent.
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Beisner William H.
Fritz Berthold
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