Device for isoelectric focusing

Electricity: measuring and testing – Using ionization effects – For analysis of gas – vapor – or particles of matter

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324 711, G01N 2762

Patent

active

044841418

ABSTRACT:
A device for detecting the isoelectric end point in an isoelectric focusing system by electronically integrating, over successive time intervals, a characteristic of the electrical input to said zone as it varies with the increasing resistance of the zone. Most conveniently, the measured characteristic is the current through the zone which, under constant impressed voltage or power, asymptotically approaches a minimum value as the end point is reached.
The measured characteristic is continually converted to a sequence of pulses indicative of the value of the characteristic. The end point is indicated when the difference between counts of these pulses over successive time intervals reaches a preselected value. At the end point, provision is made for replacement of the focusing voltage with a lower voltage to keep the ampholytes focused. A signal can be activated at the end point.

REFERENCES:
patent: 3664939 (1972-05-01), Luner et al.
patent: 3772593 (1973-11-01), Sidhu
patent: 4375401 (1983-03-01), Catsimpoolas
patent: 4433299 (1984-02-01), Kawai et al.
patent: 4443319 (1984-04-01), Chait et al.

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