Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering
Patent
1985-01-11
1986-11-11
Niebling, John F.
Chemistry: electrical and wave energy
Apparatus
Coating, forming or etching by sputtering
204299R, 2041828, G01N 2728
Patent
active
046221247
ABSTRACT:
A device for the horizontal electroblotting of electrophoretically transferred material comprising a fluid tight container having a floor, a lid and side walls extending between the floor and the lid for defining a chamber, a support assembly located in the chamber for supporting the material to be electroblotted, a first electrode provided below the support assembly and a second electrode provided above the support assembly and a barrier provided in the chamber between the first electrode and the support assembly for diverting bubbles produced by the first electrode so as to prevent the bubbles from accumulating on the support assembly during the electroblotting of the electrophoretically transferred material.
REFERENCES:
Biorad Laboratories, "Chromatography Electrophoresis Immunochemistry and HPLC", Jan. 1982, pp. 125-127 and 138-139.
Gershoni, J. M., et al., "Protein Blotting: Principles and Applications", Analytical Biochemistry, vol. 131, pp. 1-15 (1983).
Gibson, W., "Protease-Facilitated Transfer of High-Molecular-Weight Proteins During Electrotransfer to Nitrocellulose", Analytical Biochemistry, vol. 118, pp. 1-3 (1981).
McLellan, T., et al, "Serial Electrophoretic Transfers: A Technique for the Identification of Numerous Enzymes from Single Polyacrylamide Gels", Biochemical Genetics, vol. 19, pp. 647-654 (1981).
Towbin, H., et al, "Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications", Proceedings of the National Academy of Science, USA, vol. 76, pp. 4350-4353 (1979).
Kreisher John H.
McCarthy Thomas L.
Boggs Jr. B. J.
International Biotechnologies, Inc.
Niebling John F.
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