Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1979-01-22
1981-01-13
Penland, R. B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435261, 435311, 422101, C12Q 124
Patent
active
042450420
ABSTRACT:
The present invention relates to a device for harvesting cells from a plurality of wells of a standard cell culture plate. Two complementary blocks, a lower and an upper one, are adapted to be attached with each other by suitable mechanical means to form a tight fit, the lower block being provided with a channel-formed conduit attached with an external source of a washing fluid, the flow of which is controlled by valve means. The conduit is connected with a plurality of downwardly pointing tubelets which are spaced in such manner that each one of them fits into a well of the culture plate, thus providing for the possibility of introducing at will washing fluid into the wells in the culture plate. Further tubes are provided which point in the same direction, each of which is connected with an outlet at the upper face of the lower block; an O-ring is provided substantially flush with the upper face of the block at each of the outlets. A central conduit is provided in the upper block, adapted to be connected with a vacuum source. The central conduit is connected via conduits connected therewith to each of the outlets at the upper face of the lower block, forming a tight connection via the O-rings. Cell cultures from a plurality of cells may be simultaneously harvested by means of this device.
REFERENCES:
patent: 3319792 (1967-05-01), Leder et al.
patent: 3838978 (1974-10-01), Eddleman
patent: 3888770 (1975-06-01), Avital et al.
patent: 4167875 (1979-09-01), Meakin
Gideoni Nurit
Weinstein Yacob
Wolowelsky Jehoshua
Penland R. B.
Yeda Research and Development Co. Ltd.
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