Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1986-04-28
1987-11-17
Niebling, John F.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
2041828, 204299R, 204301, G01N 2728
Patent
active
047072334
ABSTRACT:
A method and device for the preparative scale recovery of a protein or other charged macromolecule by electrophoretic separation of a mixture of such macromolecules. The device is adapted to be installed between the electrodes of an electrophoretic cell and includes a gel receiving receptacle adapted to hold the end of a gel slab or gel fragments containing an electrophoretically separated macromolecule. An aperture in the base of the receptacle is covered by a first membrane which is substantially permeable to the macromolecules desired to be recovered and a second membrane substantially impermeable to the macromolecules to be recovered but permeable to electrolyte molecules in a buffer solution used in electrophoresis. The two membranes lie in spaced apart array with one another to define therebetween a recovery space. Means are provided to allow the periodic or continuous withdrawal of buffer solution and macromolecules from the recovery space.
REFERENCES:
patent: 3751356 (1973-08-01), Takeya et al.
patent: 4243507 (1981-01-01), Martin et al.
patent: 4401538 (1983-08-01), Hausfeld
Chapman Terryence
Gradiant Pty Limited
Niebling John F.
Striker Michael J.
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