Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering
Patent
1989-05-11
1991-04-16
Niebling, John F.
Chemistry: electrical and wave energy
Apparatus
Coating, forming or etching by sputtering
2041831, 435173, 435273, 935 85, 935 89, 935 93, C12N 1300
Patent
active
050079956
ABSTRACT:
A device and a method for fusing biological cells. The device is formed by a chamber having a tube for containing a cell suspension and a cover for sealing the tube. The chamber is provided with a lower electrode having a smooth and flat surface for contacting the cells precipitating in the cell suspension, and an upper electrode opposite to the lower electrode. The lower electrode forms the inner bottom surface of the tube. The upper electrode is inserted in the cover to enter the tube. The chamber is centrifuged to form the layers of cells on the lower electrode at the bottom of the tube. Then, a predetermined ac voltage is applied across the upper and lower electrodes to fuse the cells efficiently. Such a device and method are useful for the production of monoclonal antibodies or giant cells.
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patent: 4822470 (1989-04-01), Chang
Kano Tokio
Niimura Toshinobu
Suzuki Kazuo
Takahashi Yasuo
Niebling John F.
Olympus Optical Co,. Ltd.
Ryser David G.
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