Device for detecting molecules, method for detecting molecules

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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Details

C435S007100, C435S007940, C435S070210, C435S328000, C436S501000, C436S512000, C436S518000, C530S300000, C530S350000, C424S130100

Reexamination Certificate

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07659083

ABSTRACT:
A construct is provided that is capable of binding a plurality of molecules, the construct comprising a first moiety with a first molecule binding region and a first molecule non-binding region; and a second moiety with a second molecule-binding region and a second molecule-non-binding region, whereby the first binding region and second binding region are at opposite ends of the construct. Also provided is a method for detecting protein having certain amino acid sequences, the method comprising supplying a collection of proteins each with unknown amino acid sequences, contacting the collection with a moiety having a plurality of binding sites capable of binding with the protein having certain amino acid sequences so as to form a moiety-protein complex, and mixing the complex with a marker specific for the moiety in an amount sufficient to indicate existence of the complex.

REFERENCES:
Pokkuluri et al. (structure, vol. 6, pp. 1067-1073, 1998).
Padlan et al. (Proceedings of the National Academy of Science, USA vol. 86, pp. 5938-5942, Aug. 1989, Immunology).

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