Device for detecting emission light of micro-object

Optics: measuring and testing – By shade or color – With color transmitting filter

Reexamination Certificate

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Reexamination Certificate

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07489401

ABSTRACT:
In the detection of fluorescence Lf emitted by a micro-object irradiated with an excitation light Le by a semiconductor light-detecting element20,a converging microlens62for converging the excitation light Le elevating the optical density thereof and irradiating the micro-object with the light, causing the micro-object to generate fluorescence Lf due to two-photon absorption, is inserted partway along the light path of the excitation light Le. This enables the fluorescence Lf emitted by the micro-object to be detected with high sensitivity.

REFERENCES:
patent: 6201989 (2001-03-01), Whitehead et al.
patent: 2003/0222223 (2003-12-01), Kamei et al.
patent: 2004/0066805 (2004-04-01), Afzal et al.
patent: 2005/0046848 (2005-03-01), Cromwell et al.
patent: WO 03/102554 (2003-12-01), None
Toshihiro Kamei, et al, “Integrated Hydrogenated Amorphous Si Photodiode Detector for Microfluidic Bioanalytical Devices”, Analytical Chemistry, vol. 75, No. 20, Oct. 15, 2003, pp. 5300-5305.
Evan Thrush, et al., “Integrated bio-fluorescence sensor”, Journal of Chromatography A, vol. 1013, Sep. 26, 2003, pp. 103-110.
Xiaoxi Chen, et al., “A Prototype Two-Dimensional Capillary Electrophoresis System Fabricated in Poly(dimethylsiloxane)”, Analytical Chemistry, vol. 74, No. 8, Apr. 15, 2002, pp. 1772-1778.

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