Device for conducting microbiological radiorespirometric assays

Details Device for conducting microbiological radiorespirometric assays Device for conducting microbiological radiorespirometric assays

1979-12-26

1981-10-13

Warden, Robert J.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

206362, 206562, 206563, 206564, 220 23, 220 238, 422 61, 422102, C12M 120

Patent

active

042949319

ABSTRACT:
There is disclosed a device for conducting a microbiological radiorespirometric assay. The device comprises a support member which includes a plurality of pairs of chambers. One chamber in each pair contains a radioactive labeled substrate which is capable of being metabolized by at least some microorganisms to yield a radioactive gas. The other chamber in each pair contains a means for collecting radioactive gas. The two chambers in each pair are in communication with each other by means of a passageway, which opens into each of said chambers at the upper portion thereof. There is also disclosed a method for determining whether microorganisms contained in a sample material will metabolize a radioactive labeled substrate in the device. The method comprises placing said material in contact with a radioactive labeled substrate, collecting any gas which is evolved, exposing a photosensitive material to said collected gas and determining if a spot is produced on said photosensitive material. The production of a spot is indicative of the presence of radioactivity which indicates that the substrate has been metabolized by a microorganism.

REFERENCES:
patent: 2914447 (1959-11-01), Levin
patent: 3386608 (1968-06-01), Diller
patent: 3530917 (1970-09-01), Donovan
patent: 3597326 (1971-08-01), Liner
patent: 3685717 (1972-08-01), Seiferth et al.
patent: 3941660 (1976-03-01), Mirsky
patent: 3944471 (1976-03-01), Waters

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