Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1994-12-05
1996-02-06
Gitomer, Ralph J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435817, 204403, C12Q 102, G01N 2700
Patent
active
054895154
ABSTRACT:
A device for analyzing the metabolism of cells includes a supporting base which carries a pH measuring electrode, a control electrode designed for proton exchange with the liquid, a reference electrode, and a counterelectrode, and which, together with at least one other part, confines a hollow space containing the cells and a liquid. The electrodes are connected to electronic circuit elements. In the course of an analysis the pH value of the liquid is determined by means of the pH measuring electrode and the reference electrode. Between the control electrode and the counterelectrode an electric current is passed through the liquid and controlled such that the pH of the liquid is maintained at a constant preset value beneficial to the cells, by proton exchange with the control electrode. In addition, the amount of charge required for generating the current during the measuring period is determined to obtain a measure for the intensity of the metabolic process and thus for the vitality of the cells.
REFERENCES:
patent: 4591550 (1986-05-01), Hafeman
patent: 4915812 (1990-04-01), Parce
Parce J., Detection of Cell-Affecting Agents . . . Science 246 (1989) pp. 243-247.
Owicki J., Continuous Monitoring of Receptor . . . Proc Natl Acad Sci 87 (1990) pp. 4007-4011.
Owicki J., Biosensors Based on the Energy Metabolism . . . Bioscensors & Bioelectronics 7 (1992) 255-272.
Grattarola M., On-Like Extra Cellular pH . . . STN Pharma Sciences (1993) 3 (1) pp. 31-34.
Hatschek Rudolf
Heitz Erich W. F.
AVL Medical Instruments AG
Gitomer Ralph J.
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