Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1997-10-16
1999-11-02
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 34, 435177, 435180, 435395, 435402, 4354821, 4352871, 4352891, C12Q 102, C12Q 104, C12N 1102, C12N 1108
Patent
active
059768266
ABSTRACT:
A device is provided for adhering cells in a specific and predetermined position. The device comprises a plate defining a surface and a plurality of cytophilic islands that adhere cells which are isolated by cytophobic regions to which cells do not adhere and further is contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM). Further, the cytophobic regions are wide enough such that less than 10 percent of the cells adhered to the cytophilic islands are allowed to form bridges across the cytophobic regions and contact each other. The device is used in a method for culturing cells on a surface or in a medium and also for performing cytometry. Furthermore, the device is used in immobilization of cells at a surface and for controlling the shape of a cell.
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Ingber Donald E.
Kumar Amit
Lopez Gabriel P.
Singhvi Rahul
Stephanopoulos Gregory N.
Children's Medical Center Corporation
Massachusetts Institute of Technology
Naff David M.
President and Fellows of Harvard College
Ware Deborah K.
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