Device, composition and method for identifying microorganisms

Textiles: fiber preparation

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Details

1951035M, 195140, 195127, C12K 110

Patent

active

041294831

ABSTRACT:
In accordance with the invention there is provided means for identifying or testing a microorganism by growing a culture of the microorganism in contact with a composition containing: (1) an oxidation-reduction indicator, preferably a tetrazolium compound that undergoes a change in color upon reduction to a formazan compound; (2) a biodegradable compound which when catabolized by the microorganism will engender a reduction of the tetrazolium compound to the formazan compound; (3) a buffer to maintain the pH of the mixture at a level which enables culture growth without itself either engendering or preventing reduction of the tetrazolium compound to the formazan compound; and (4) a nutrient for the microorganism in a concentration sufficient to support culture growth without itself engendering reduction of the tetrazolium compound to the formazan compound. Further in accordance with the invention, there is provided a compartmentalized container to which the microorganism culture is admitted, each compartment thereof containing a composition as described but with the composition in each compartment differing from the others by way of the second-mentioned compound included. The color changes, or lack of color changes, in the various compartments are indicative of the catabolic behavior of the microorganism and hence of the type of microorganism itself.

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patent: 3415718 (1968-12-01), Forkman et al.
patent: 3597321 (1971-08-01), Kronish et al.
patent: 3728228 (1973-04-01), Durantz
patent: 3776818 (1973-12-01), Khan
patent: 3832288 (1974-08-01), Rollender et al.
patent: 3881993 (1975-05-01), Freake et al.

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