Device and system for reversibly trapping and isolating...

Details Device and system for reversibly trapping and isolating... Device and system for reversibly trapping and isolating...

2006-02-14

2006-02-14

Therkorn, Ernest G. (Department: 1723)

Liquid purification or separation

With means to add treating material

Chromatography

C210S502100, C210S635000, C210S638000, C210S656000, C422S070000, C422S105000

Reexamination Certificate

active

06998041

ABSTRACT:
A device and system for reversibly trapping and isolating carbohydrate moieties. The apparatus includes an aldehyde-containing trapping material, a trapping means into which the aldehyde-containing trapping material is packable and through which hydrazine-activated carbohydrate-containing samples are passable, and additional hydrazine to release hydrazine-activated carbohydrates from the trapping material. As a result, all remaining non-carbohydrate material is not bound to the apparatus.

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Kett, et al., Heterocylic derivatives of sugards; An NMR study of the formation of 1-glycosyl-3, 5-dimethyl-1 H-pyrazoles from hydrazones, Carbohydrate Research 299 (1997) 129-141.
Bendiak, et al., Hydrazinolysis-N-Reacetylation of Glycopeptides and Glycoproteins. Model Studies Using 2-Acetamido-1-N (L-Aspart-4-Oyl)-2-Deoxy-B-D-Glucopyranosylamine, Carbohydrate Research, 144 (1985) 1-12.
Bendiak, Preparation, conformation, and mild hydrolysis of 1-glycosyl-2-acetylhydrazines of the hexoses, pentoses, 2-acetamido-2-deosyhexoses, and fucose, Carbohydrate Research 304 (1997) 85-90.

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