Chemistry: analytical and immunological testing – Including sample preparation – Volumetric liquid transfer
Patent
1998-08-21
2000-10-31
Le, Long V.
Chemistry: analytical and immunological testing
Including sample preparation
Volumetric liquid transfer
436518, 422100, 422103, 422131, 422134, B01J 1900
Patent
active
061401331
DESCRIPTION:
BRIEF SUMMARY
The invention relates to a device for the synthesis of macromolecules, especially in sequentially homologous series, having at least one housing member comprising a number of reaction channels and having a further housing member comprising sealing portions with which the reaction channels can be covered over, in which device between the housing members a carrier material, which can be covered in synthesis zones by reaction channels, can be positioned in a number of different arrangements relative to the reaction channels.
The invention relates also to a process for the synthesis of macromolecules, especially in sequentially homologous series, in which reaction solutions are conveyed in synthesis zones over a carrier material by way of a number of reaction channels.
Such a device and such a process are known from WO 95/11748. The generic device has a number of reaction channels provided in a housing member, it being possible for a carrier material to be inserted between the housing member comprising the reaction channels and a further housing member comprising sealing portions that cover over the reaction channels. For the synthesis of macromolecules, different reagent solutions are conveyed through the reaction channels in a sequential order that is also variable.
After carrying out a succession of first synthesis steps in a first position, after which there are present in each of the synthesis zones covered by reaction channels macromolecules having first sequences of structural units, in further positioning steps the reaction channels are so rotated, for example through 90.degree., and possibly subsequently displaced linearly, that in the resulting new position they cover in cellular regions synthesis zones that were previously covered by other reaction channels. In a succession of further synthesis steps, further sequence portions of structural units can be added to the macromolecules formed in the cellular regions during the first synthesis steps.
Although the generic device and the generic process allow the formation of a two-dimensional field of macromolecules with a combination of portions having different sequences, the synthesis of macromolecules having different sequences is, of course, very complex in terms of apparatus because it requires a large number of reaction channels to be acted on by different reagent solutions, or alternatively, with an acceptable number of positioning steps and the proviso of as high as possible a sequence variation, the quantitative yield is relatively low because of the consequently relatively small cellular regions.
The problem underlying the invention is therefore to provide a device and a process of the kind mentioned at the outset that allows the synthesis of macromolecules having different sequences in a high yield using relatively simple apparatus.
The problem is solved in accordance with the invention, in a device of the kind mentioned at the outset, by the provision of intermediate channels by which a number of reaction channels are connected to one another.
The problem is solved in a process of the kind mentioned at the outset by the arrangement of reaction channels, which are connected to one another by intermediate channels, and the carrier material in different relative positions in different synthesis steps to cover synthesis zones.
The displacement of a carrier material relative to a chain of reaction channels that are connected to one another by intermediate channels by a distance that corresponds to the periodic spacing of the reaction channels or to a multiple thereof has the result, in the case where synthesis steps are carried out in succession at the individual positions, that oligomers having different sequences of structural units are formed in synthesis zones of large area, only one reaction solution having a single specific composition having been conveyed through the linked reaction channels in each synthesis step. As a result of the displacement of the reaction channels relative to, for example, directly adjacent synthesis zones that have a
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Bader Raoul
Haener Robert
Hinz Michael
Seliger Hartmut
Le Long V.
Novartis AG
Wildman David E.
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