Device and methods for subdividing and filtering gel...

Liquid purification or separation – Processes – Liquid/liquid solvent or colloidal extraction or diffusing...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C204S456000, C204S462000, C210S650000, C210S787000, C241S001000, C241S024100, C436S178000, C436S180000

Reexamination Certificate

active

06916423

ABSTRACT:
An apparatus and method for mincing a gel includes a gel mincing tube and a mesh material. The mesh material extends across the end of the tube. To subdivide a gel using the mincing apparatus, a gel is placed upon the mesh material in the mincing tube, the mincing tube, mesh material and the gel are spun in a centrifuge, forcing the gel through the mesh material so that the gel is subdivided into generally uniform smaller fragments. The mesh material may be secured to a tube in the form of a nesting tube. The nesting tube nests within the opening of a recovery vessel. The mesh material may be placed in series with a conditionally porous membrane in the nesting tube. Centrifuging the nesting tube and the recovery vessel subdivides gel material into fragments by forcing the gel through the mesh material. The gel subsequently falls upon the membrane, and may be treated on the membrane to extract or otherwise treat analytes in the gel material.

REFERENCES:
patent: 3763879 (1973-09-01), Jaworek
patent: 4734192 (1988-03-01), Champion et al.
patent: 4948561 (1990-08-01), Hinckley et al.
patent: 5552325 (1996-09-01), Nochumson et al.
patent: 5601711 (1997-02-01), Sklar et al.
patent: 5833860 (1998-11-01), Kopaciewicz et al.
patent: 5853586 (1998-12-01), Valus et al.
patent: 6048457 (2000-04-01), Kopaciewicz et al.
patent: 6103195 (2000-08-01), Shukla et al.
patent: 6200474 (2001-03-01), Kopaciewicz et al.
patent: 6221655 (2001-04-01), Fung et al.
patent: 6291249 (2001-09-01), Mahant et al.
patent: WO 99/39010 (1999-08-01), None
Pluskal et al., “High Throughput In-Gel Peptide Digestion and Microscale Sample Preparation for MALDI-TOF MS Analysis”, one (1) page, presented at Conference, May 14-17, 2001, Philadelphia, PA.
Retamal et al., “Protein Purification from Polyacrylamide Gels by Sonication Extraction”,Analytical Biochemistry(1999) 268, pp. 15-20.
Ihara et al., “Recovery of Polypeptides from Polyacrylamide Gels by Electrophoretic Elution in a Centrifugation Concentrator”,Analytical Biochemistry(1987) 166, pp. 349-352.
Castellanos-Serra et al., “Fast Passive Elution of DNA from Zinc-Imidazole Negatively Stained Polyacrylamide Gels”,Analytical Biochemistry(1998) 257, pp. 227-228.
Kobayashi et al., “Isolation of Enzymes from Polyacrylamide Disk Gels by a Centrifugal Homogenization Method”,Analytical Biochemistry(1985) 145, pp. 351-353.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Device and methods for subdividing and filtering gel... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Device and methods for subdividing and filtering gel..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Device and methods for subdividing and filtering gel... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3412065

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.