Device and methods for multigradient directional cooling and war

Refrigeration – Processes – Treating an article

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

62 64, 62 65, 62374, 62380, F25D 1306

Patent

active

058732543

ABSTRACT:
A device for controlled freezing and warming of a biological sample, and freezing and thawing protocols for which the device is well suited. The device establishes a laterally varying thermal gradient and provides a mechanism for moving the sample along the thermal gradient at a controlled rate of speed. The sample is moved along the thermal gradient at a rate of speed that provides a variable cooling rate or a variable warming rate in accordance with the appropriate protocol. The device also allows continuous seeding of the sample through the freezing process at the exact freezing point of the solution. Real time monitoring and video imaging of the freezing process enable fine tuning of the thermodynamic parameters for improved control.

REFERENCES:
patent: 4972681 (1990-11-01), Lofkvist
patent: 5630321 (1997-05-01), Miller
Arav, A., "Vitrification of Oocytes and Embryos", found in Embronic Development and Manipulation, Eds. Lauria & Gandalfi, Portland Press (1992), pp. 255-264.
Arav, A. et al, "Cyropreservation of Bovine Semen with Directional Solidification", Cyrobiology, Atlanta Meeting, (1993).
Rubinsky, B. et al, "Cyropreservation of Oocytes Using Directional Cooling and Antifreeze Glycoprotiens", Cyro-Letters, 12:93-106 (1991).
Rubinsky, B. et al, "A Cryomicroscope Using Directional Solidification for the Controlled Freezinf of Biological Material", Cryobiology, 22:55-68 (1985).
Brower, W.E. et al, "An Hypothesis for Survival of Spermatozoa Via Encapsulation During Plane Front Freezing", Cryobiology, 18: 277-291 (1981).
Beckmann J. et al., "Redefining Cooling Rate in Terms of Ice Front Velocity and Thermal Gradient: First Evidence of Relevance to Freezing Injury of Lymphocytes", Cryobiology, 27:279-287 (1990).
Hubel A. et al., "Survival of Directionally Solidified B-Lynphoblasts under Various Crystal Growth Conditions", Cryobiology, 29:183-198 (1992).
Arav A. et al., "The Role of Thermal Hysteresis Proteins During Cropreservation of Oocytes and Embryos", Theriogenology, 41:107-112 (1994).
Arav A et al., "Recent Developments in Cyropreservation of Stallion Semen with Special Emphasis on Thawing Procedure Using Thermal Hysteresis Proteins", Zygote 2(Nov. 1994) pp. 379-382.
Arav A. et al., "Arav A. et al., Crioconservazione Di Oociti Immaturi Di Maiale Mediante Vitrificzione", Estratto Da Selezione Veterinaria, 1991, vol. XXXII, n. 1 bis (Abs in English).
Arav A. et al., "Osmotic and Cytotoxic Study of Vitrification if Immature Bovine Oocytes", J. Of Repro. and Fert., (1993) 99:353-358.
Koebe et al, "Temperature Gradients in Freezing Chambers of Rate-Controlled Cooling Machines" Cryobiology, 30, 349-352 (1993).
Fiser, P.S. et al., "The Effect of Induced Ice Nucleation (Seeding) on the Post-Thaw Motility and Arosomal Integrity of Boar Spermatozoa", Animal Repro. Science, 24:293-304 (1991).
Luyet, et al, "A Critical Temperature Range Apparently Characterized By Sensitivity of Bull Semen To High Freezing Velocity", Biodynamica, vol. 7, No. 152, pp. 281-292 (1955).
Weitze et al., "Fertility of Frozen Boar Semen: Influence of Packaging, Number of Inseminations, and Seminal Plasma", no publishing info given.
Shabana et al, "Fluorometric Analysis of Transient Solute Redistribution Fields During Freezing of Aqueous Solutions" Advances in Cryobiology and Cryomedicine (1995).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Device and methods for multigradient directional cooling and war does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Device and methods for multigradient directional cooling and war, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Device and methods for multigradient directional cooling and war will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-298204

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.