Device and method of assaying for trace mounts of proteins

Details Device and method of assaying for trace mounts of proteins Device and method of assaying for trace mounts of proteins

1988-06-06

1990-10-02

Warden, Robert J.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436 66, 436166, 436169, 422 56, G01N 3348

Patent

active

049607103

ABSTRACT:
A new and improved method and composition for determining the presence and concentration of low to trace amounts of proteins, such as albumin, in a test sample, such as urine. The method includes using a reagent composition capable of interacting with low to trace amounts of proteins to produce a visually or instrumentally detectable and/or measurable response. The method can be used in wet assays or in dry test strip assays, wherein the reagent composition is incorporating into a carrier matrix. The new and improved reagent composition, comprising a dye, such as a polyhydroxybenzenesulfonephthalein-type indicator, like pyrocatechol violet; a tungstate, such as ammonium tungstate; and, if necessary, a suitable buffer, is incorporated into the carrier matrix to provide sufficient sensitivity to low to trace protein levels and sufficient color resolution between low to trace protein levels, thereby affording an accurate and trustworthy protein assay of test samples having a low protein concentration.

REFERENCES:
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patent: 4143080 (1979-03-01), Harders et al.
patent: 4268270 (1981-05-01), Gabbay et al.
Fujita et al., Bunsekikagaku, vol. 32 (12), pp. 379-386, 12-1983.
Watanabe et al., Clin. Chem., 8-1986 (32) 8, pp. 1551-4.
Lebedeva et al., Vestn. Leningr. Div., Ser. 4: Fitzjkhim, Kohara, Kitakyushu Kogyokoto Semmon Oakko Kenkyu Hokoku, 1978, vol. 11, p. 165-170.
Akimova et al., Zh. Anal. Khim, 1975, vol. 30(3), pp. 533-39.

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