Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1993-08-04
1994-09-20
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
435 4, 435 29, 435 35, 435287, 435297, 435299, 435301, 435311, 435807, 73 232, 73 2901, 73 2904, 73 6454, C12M 134, C12M 100, C12N 1100, C12Q 102
Patent
active
053488849
ABSTRACT:
Selective detection of microorganisms is achieved by a screening technique in which radioactively labeled, low-molecular-weight metabolites produced by microorganisms to be detected reach an adsorption medium through a semipermeable medium which passes the labeled metabolites but blocks the labeled incubation medium. The adsorption medium is then subject to analysis, such as autoradiography, to detect the presence of the labeled metabolites. An analytical assembly for such detection includes a filter membrane support for growing microorganisms in discrete colonies and for holding a radioactively labeled incubating medium applied thereon, a semipermeable filter medium covering the support having the grown microorganisms and the incubating medium for passing low-molecular-weight, labeled metabolites produced by microorganisms of interest while blocking the incubating medium, and a cohesive layer of an adsorption medium conformably disposed with the filter membrane support and the semipermeable medium and being capable of adsorbing the labeled metabolites which have traversed the semipermeable medium during incubation of the assembly. Following incubation of the assembly, the adsorption medium layer is transferable for analysis, e.g., by autoradiography, to detect the presence of labeled metabolites produced by microorganisms of interest and to identify specific colonies of the microorganisms producing the radioactively labeled metabolite.
REFERENCES:
patent: 3929582 (1975-12-01), Kellner
patent: 4421849 (1983-12-01), Breuker
Ullmann's Encyclopedia of Industrial Chemistry, 5th Ed., vol. B3, VCH Verlagsgesellschaft mbH, 1988, pp. 9-8 and 9-9.
Austin "Shreve's Chemical Process Industries", Fifth Edition, McGraw Hill Book Company.
Lim et al., Applied and Environmental Microbiology, Feb. 1977, pp. 328-333 vol. 33, No. 2.
Patent Abstracts of Japan, vol. 9, No. 309, Dec. 5, 1985.
T. A. McMeekin et al., "Detection of Volatile Sulfide-Producing Bacteria Isolated From Poultry-Processing Plants", Applied and Environmental Microbiology, vol. 35, No. 6, Jun. 1978.
J. L. Firmin et al., "The Biochemical Pathway for the Breakdown of Methyl Cyanide (Acetonitrile) in Bacteria," Biochemical Journal, vol. 158 (1976), pp. 223-229.
B. R. Bochner et al., "Sensitive Fluorographic Detection of .sup.3 H and .sup.14 C on Chromatograms Using Methyl Anthranilate as a Scintillant," Anal. Biochem., vol. 131 (1984), pp. 510-515.
R. A. Laskey, "Radioisotope Detection by Fluorography and Intensifying Screens", Amersham, United Kingdom, Review 23, 1984.
H. Kulla et al., "Interference of Aromatic Sulfo Groups in the Microbial Degradation of the Azo Dyes Orange I and Orange II," Arch. Microbiol., vol. 135 (1983), pp. 1-7.
Lonza Ltd.
Williams Jane
Wityshyn Michael G.
LandOfFree
Device and method for the detection of microorganisms which prod does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Device and method for the detection of microorganisms which prod, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Device and method for the detection of microorganisms which prod will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2429814