Chemistry: analytical and immunological testing – Process or composition for determination of physical state... – Of crystal or crystalline material
Patent
1995-04-17
1997-06-24
Soderquist, Arlen
Chemistry: analytical and immunological testing
Process or composition for determination of physical state...
Of crystal or crystalline material
436 86, 4222451, 117 70, 117201, 117901, C30B 700, G01N 3100
Patent
active
056416814
ABSTRACT:
A device and method for detecting optimum protein crystallization conditions and for growing protein crystals in either 1g or microgravity environments comprising a housing defining at least one pair of chambers for containing crystallization solutions. The housing further defines an orifice therein for providing fluid communication between the chambers. The orifice is adapted to receive a tube which contains a gelling substance for limiting the rate of diffusive mixing of the crystallization solutions. The solutions are diffusively mixed over a period of time defined by the quantity of gelling substance sufficient to achieve equilibration and to substantially reduce density driven convection disturbances therein.
The device further includes endcaps to seal the first and second chambers. One of the endcaps includes a dialysis chamber which contains protein solution in which protein crystals are grown. Once the endcaps are in place, the protein solution is exposed to the crystallization solutions wherein the solubility of the protein solution is reduced at a rate responsive to the rate of diffusive mixing of the crystallization solutions. This allows for a controlled approach to supersaturation and allows for screening of crystal growth conditions at preselected intervals.
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Soderquist Arlen
The United States of America as represented by the Administrator
Welborn Brian S.
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