Device and method for providing a hybridization chamber and...

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

Reexamination Certificate

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Details

C422S105000, C435S286700, C435S288300

Reexamination Certificate

active

07459306

ABSTRACT:
A device, appropriate process units and/or systems for providing a gap-shaped hybridization space to hybridize nucleic acid samples, proteins or tissue sections over a slide. Said items of device are able to move in relation to the slide and comprise an annular sealing surface to seal the hybridization space by application to a surface of the slide. Said items of device additionally comprise lines to introduce media to and remove them from the hybridization space. Said items of device furthermore comprise a specimen supply line and preferably an agitation device to move fluids in relation to the samples immobilized on the surface of the slide. The devices according to the invention are characterized in that they limit a hybridization space that features relief structures to conduct and/or block air bubbles on at least one surface of the device and the slide directed at the interior of this hybridization space. According to the method of the invention, air bubbles present in the hybridization space are conducted and/or blocked by these relief structures.

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patent: 5951952 (1999-09-01), O'Beat et al.
patent: 6258593 (2001-07-01), Schembri et al.
patent: 6946287 (2005-09-01), Streit et al.
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patent: 2006/0003440 (2006-01-01), Streit et al.
patent: 0 785 433 (1997-07-01), None
patent: 1 260 265 (2002-11-01), None
patent: WO 03/106033 (2003-12-01), None
Applied Powe Products-Product Line Listing. Sep. 12, 2002 p. 3.

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