Device and method for preparative electrophoresis

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

Reexamination Certificate

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C204S600000, C210S637000

Reexamination Certificate

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07449093

ABSTRACT:
Method and appliance for carrying out membrane electrophoresis using microfiltration or ultrafiltration membranes, wherein the electroosmotic flow which develops in the membrane pores is reduced or offset by pressure superposition. The appliance for the membrane electrophoresis comprises at least an at least quadrupartite separation chamber (7), having at least one diluate space (16) and one concentrate space (17) and also a cathode space (18) and an anode space (21) having electrodes as anode (19) and cathode (20), with the individual spaces (16, 17, 18, 21) being separated from each other by ultrafiltration or microfiltration membranes (14, 15); feed lines (22) and discharge lines (23) for the diluate, feed lines (24) and discharge lines (25) for the concentrate, optionally feed lines (26) and discharge lines (27) for the electrode rinsing solution, and also a device (8; 10) or (9; 11) for pressure regulation by means of which a pressure difference, in particular of at least 3 kPa, can be generated between the diluate space (16) and the concentrate space (17).

REFERENCES:
patent: 3989613 (1976-11-01), Gritzner
patent: 4043895 (1977-08-01), Gritzner
patent: 4043896 (1977-08-01), Ahlgren
patent: 4180451 (1979-12-01), McRae
patent: 4243507 (1981-01-01), Martin et al.
patent: 4608147 (1986-08-01), Clad
patent: 4758320 (1988-07-01), Sanchez et al.
patent: 5087338 (1992-02-01), Perry et al.
patent: 6270672 (2001-08-01), Turecek et al.
patent: 2003/0019753 (2003-01-01), Ogle et al.
patent: 33 37 669 (1985-05-01), None
patent: 36 26 953 (1987-03-01), None
patent: 0 369 945 (1990-05-01), None
patent: WO 94/11728 (1994-05-01), None
Galier et al.,J. Membr. Sci. 194 (2001) 117-133.

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